Study type: Medical/biological study (experimental study)

Magnetic field exposure enhances mRNA expression of sigma 32 in E. coli. med./bio.

Published in: J Cell Biochem 1998; 68 (1): 1-7

Aim of study (acc. to author)

To examine the stress response in Escherichia coli exposed to a magnetic field.

Background/further details

The examined parameter was the transcription of Sigma 32 (σ32), a protein that is essential for the transcription initiation of heat shock protein genes. It enhances the RNA polymerase binding probability to promoter sides on DNA. Therefore sigma 32 represents a good marker of stress response in Escherichia coli.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 60 Hz
Exposure duration: 15 min

Exposure 1

Main characteristics
Frequency 60 Hz
Type
Waveform
Exposure duration 15 min
Exposure setup
Exposure source
Setup Helmholtz coils mounted on a wooden frame; culture flasks placed in water-jacketed cylinder that was surrounded by the dual-filament-wound coils
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 1.1 mT effective value measured - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

The intracellular level of sigma 32 mRNA was significantly enhanced following the exposure to magnetic field.

Study character:

Study funded by

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