Transposable elements are found in all organisms and it is believed that they play an important role in evolution. For some organisms it has been shown that transposition activity can be induced by stressing factors (as heat shock and UV radiation).
Controls were performed with bacterial SURE strains that have been additionally transformed with different plasmids for the inductive over-expression of the heat shock proteins DnaK/DnaJ (HSP70/HSP40) and GroEL/ES. Since DnaK and GroEL are known to be involved in homologous recombination, they are possible participants in mediating the Tn5 transposition stimulation.The controls were incubated for 3 hours in a 37°C water bath.
Exposure duration: continuous, 1 h
|magnetic flux density||1.2 mT||peak value||measured||unspecified||-|
The data showed that under the conditions defined a magnetic field of 50 Hz stimulated the transposition activity of Tn5 and such stimulation was mediated through the induction of the heat shock protein DnaK/J synthesis.