Study type:
Medical/biological study
(experimental study)
Effects of the static and ELF magnetic fields on the neuronal population activity in Morimus funereus (Coleoptera, Cerambycidae) antennal lobe revealed by wavelet analysis
med./bio.
The speciesMorimus funereus is classified as "vulnerable" by the International Union for Conservation of Nature and Natural Resources (IUCN). Hence, it is important to understand how different ecological factors such as magnetic fields could affect the nervous system of this beetle. An additional goal of this study was to test whether the wavelet transformation is suitable to measure the potentialexposureinduced effects on the neuronalpopulation activity of insects. Three experiments were conducted: 1.) exposure to a static magnetic field (n=7), 2.) exposure to a sine wave50 Hzmagnetic field (n=12) and 3.) exposure to a square wave50 Hzmagnetic field (n=10). In the first experiment, a 5 minutes baseline was recorded before the exposure period, followed by a 5 minutes recording during exposure and a 5 minutes after-exposure recording. The experiments 2 and 3 consisted of 3 exposuresessions with exposure durations of 5, 10, and 15 minutes. Recordings were performed 5 minutes before the first exposure (baseline) as well as immediately after the first exposure period (5 min) and 35 min later. Immediately after the "35 minutes later recording" started the next exposure period (10 min) and 35 min later the next recording. Subsequently, a 15 min exposure period started, which was 35 min later followed by the last recording.
Endpoint
effects on the neurological system: neuronal activity of antennal lobe of beetles
Distance between exposed object and exposure source
1 cm
Setup
magnetic field was generated by a Teflon coil (length 70 mm, diameter 30 mm) which was coiled by enameled copper wire; a soft iron core (diameter of 8 mm) was placed in the middle of the cylinder, so it represents a single unit; magnet was connected to the power amplifier and the frequency generator, which enabled generation of different waveforms; sine wave and square wavecurrent (0.3 A, 12 V, 50 Hz) flows through the magnet inducing MF of 19 mT on the top of the core; an oscilloscope was used to check the waveform accuracy; magnet was placed 1 cm from the left antenna of the beetle
rise time of the square was about 2.2 ms, the root mean of the amplitude of the square signal was 630 mV, width of impulses was 5 ms, and period was 20 ms
Exposure setup
Exposure source
same setup as in E2
Distance between exposed object and exposure source
Ministry of Education, Science and Technological Development, Serbia
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