この研究は、カミキリムシ（Coleoptera, Cerambycidae）の成虫に、静磁界（SMF：2 mT）およびELF磁界（ELF MF：50Hz、2 mT）のばく露を与え、触角葉でのニューロン集団活動を表す局所フィールド電位（LFPs）を測定して、ウェーブレット解析した。その結果、ELF MFばく露では、正弦波または方形波で、対照群に比べ、4-8Hz帯の相対ウェーブレットエネルギー（RWE）が有意に上昇し、1-2Hz帯のRWEが有意に減少した；64-128Hz帯のRWEが方形波で減少した；SMFでは､5分間ばく露で何の変化も見られなかった、と報告している。
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The species Morimus funereus is classified as "vulnerable" by the International Union for Conservation of Nature and Natural Resources (IUCN). Hence, it is important to understand how different ecological factors such as magnetic fields could affect the nervous system of this beetle.
An additional goal of this study was to test whether the wavelet transformation is suitable to measure the potential exposure induced effects on the neuronal population activity of insects.
Three experiments were conducted: 1.) exposure to a static magnetic field (n=7), 2.) exposure to a sine wave 50 Hz magnetic field (n=12) and 3.) exposure to a square wave 50 Hz magnetic field (n=10). In the first experiment, a 5 minutes baseline was recorded before the exposure period, followed by a 5 minutes recording during exposure and a 5 minutes after-exposure recording. The experiments 2 and 3 consisted of 3 exposure sessions with exposure durations of 5, 10, and 15 minutes. Recordings were performed 5 minutes before the first exposure (baseline) as well as immediately after the first exposure period (5 min) and 35 min later. Immediately after the "35 minutes later recording" started the next exposure period (10 min) and 35 min later the next recording. Subsequently, a 15 min exposure period started, which was 35 min later followed by the last recording.
No significant influence of the static magnetic field on the neuronal population activity in the antennal lobe was observed.
Exposure to extremely low frequency magnetic fields induced significant changes in the slow (1-2 Hz, 4-8 Hz) and in the fast (64-128 Hz) frequency bands of the local field potentials from the antennal lobe compared to the corresponding baseline: In the second experiment (sine wave), the relative wavelet energy in the range of 1-2 Hz was significantly decreased after the repeated and prolonged exposures, while it was increased in the 4-8 Hz frequency band immediately after the first exposure as well as after repeated exposures. In the third experiment (square wave), the relative wavelet energy of the 4-8 Hz frequency band was significantly increased immediately after the first exposure as well as after repeated exposures. In the 1-2 Hz range, the relative wavelet energy was significantly decreased only after repeated exposures and in the 64-128 Hz range it was significantly decreased immediately after the first exposure and after repeated exposures.
The authors conclude that extremely low frequency magnetic fields could affect the neuronal population activity in the antennal lobe of Morimus funereus and that wavelet transformation presents a valuable tool for measuring these changes.
