Study type: Medical/biological study (experimental study)

Effect of microwave oven induced mild hyperthermia on bone viability and strength med./bio.

By: Liebergall M, Abu-Sneineh CH, Eylon S, Mendelson S, Segal D, Simkin A
Published in: Clin Orthop Relat Res 2000; (372): 272-279
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Aim of study (acc. to author)

To evaluate bone cell viability and bone mechanical function after hyperthermal treatment at several combinations of heating using a regular home microwave oven.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 2.45 GHz
Exposure duration: 10, 30, 40, 60, 90 s
Exposure 2: 2.45 GHz
Exposure duration: 10, 20, 30, 60, 90 s
bones heated in saline

Exposure 1

Main characteristics
Frequency 2.45 GHz
Type
Exposure duration 10, 30, 40, 60, 90 s
Exposure setup
Exposure source
Setup Bones were heated in empty petri dishes placed in the center of the oven.
Parameters
Measurand Value Type Method Mass Remarks
power 1.5 kW maximum calibration - -

Exposure 2

Main characteristics
Frequency 2.45 GHz
Type
Exposure duration 10, 20, 30, 60, 90 s
Additional info bones heated in saline
Exposure setup
Exposure source
Setup Bones were heated in 35 mL of normal saline in petri dishes placed in the center of the oven.
Parameters
Measurand Value Type Method Mass Remarks
power 1.5 kW maximum calibration - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

It was found that complete eradication of all viable cells in rat bone could be achieved with minimal reduction in mechanical function. When the cells were evaluated histologically by lactate dehydrogenase activity, complete bone cell death occurred after 60 seconds of heating in an empty petri dish and after 30 seconds in a petri dish containing normal saline. Mechanical stiffness and strength of the bones showed no decrease after this heating.
Microwave oven induced hyperthermia eradication of viable cells without significant damage to the mechanical properties may have clinical relevance in limb salvage tumor surgery.

Study character:

Study funded by