Study type: Medical/biological study (experimental study)

Primary DNA damage in human blood lymphocytes exposed in vitro to 2450 MHz radiofrequency radiation. med./bio.

Published in: Radiat Res 2000; 153 (4): 479-486

Aim of study (acc. to editor)

To study primary DNA damge in human blood lymphocytes exposed to 2450 MHz radiofrequency radiation.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 2.45 GHz
Modulation type: pulsed
Exposure duration: continuous for 2 h

Exposure 1

Main characteristics
Frequency 2.45 GHz
Type
Charakteristic
Exposure duration continuous for 2 h
Modulation
Modulation type pulsed
Pulse width 10 µs
Duty cycle 10 %
Repetition frequency 10 kHz
Exposure setup
Exposure source
Distance between exposed object and exposure source 1.75 m
Chamber The anechoic room of 5.2 x 3.35 x 3.35 m was maintained at 22 ± 1°C. A Plexiglas (0.6-cm thick) box with two compartments and outside dimensions of 24.5 x 21.0 x 3.7 cm was placed in a tightly fitting pressed-foam box of 27 x 22 x 7 cm which was positioned directly under the horn antenna.
Setup Four T-25 tissue culture flasks were positioned in the open upper compartment of 1.2 cm height, three of them containing 6 ml of whole blood, the fourth one being used for temperature monitoring. The compartment was filled with warm water up to the height of the medium in the flasks. The closed lower compartment was circulated with warm water to maintain the liquid in the flasks at 37°C.
Sham exposure A sham exposure was conducted.
Additional info The pressed-foam box for simultaneous sham exposure was placed at the far end of the anechoic room away from the horn. Control samples were incubated for 2 h minus 5 min and in the end exposed to 50 cGy of ionizing radiation from a 137Cs γ-ray source at a dose rate of 1.008 cGy/min.
Parameters
Measurand Value Type Method Mass Remarks
power 21 W - - - net forward power
power density 5 mW/cm² mean measured - ±7%
SAR 2.135 W/kg mean calculated - ± 0.005 W/kg
SAR 5.015 W/kg maximum calculated - see add. information

Reference articles

  • Vijayalaxmi et al. (1997): Proliferation and cytogenetic studies in human blood lymphocytes exposed in vitro to 2450 MHz radiofrequency radiation.

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

No evidence for induction of DNA single-strand breaks and alkali-labile lesions in human blood lymphocytes exposed to pulsed-wave 2450 MHz radiofrequencey radiation was found, either immediately or at 4h after exposure.

Study character:

Study funded by

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