To study the influence of microwave irradiation on the conformation of λ phage (lambda phage) DNA. The DNA samples were subjected to restriction fragmentation by EcoRI and BamHI (i.e. the restriction enzymes cut/digest the DNA and produce fragments of different lengths).
Exposure duration: 2, 4, 8, 12, 16, and 20 sec
No parameters are specified for this exposure.
EcoRI fragmentations of microwaved DNA samples yielded three additional fragments, while BamHI fragmentations did not yield any additional fragments. Mobilities of the BamHI fragments from the microwaved DNA samples were slower and the bands were broader in comparison to those from native samples. These altered restriction patterns were attributed to the anomalies of conformation in DNA resulting from single strand breaks and localized strand separations induced by microwave exposure.