Isolated neutrophils were primed by a chemotactic peptide N-formyl-methionyl-leucyl-phenylalanine (fMLP, 10 nM for 20 min), and then activated by 1 µM fMLP.
Different sets of intact and treated cells were irradiated simultaneously: (1) intact cells and primed cells; (2) primed cells and the cells treated initially with one of the inhibitors and then 1 min later with the priming agent; (3) intact cells, primed cells, and the cells treated initially with one of the inhibitors and then 1 min later with the priming agent. All cells were activated with 1 µM fMLP.
|Distance between exposed object and exposure source||0.4 m|
|Setup||mini dishes with cells were placed in the mini-dish holders inside the incubator.|
|Additional info||12 dishes, 6 with irradiation placed inside the incubator in a sample holder fixed on a horizontal polyurethane partition with a thickness of 10 mm. A hole 40 mm in diameter made in the partition exactly over the antenna. A holder with non-irradiated dishes was located 80 mm from the sample holder.|
Preliminary millimeter wave irradiation of the unprimed cells at 20°C did not act on the ROS production induced by the chemotactic peptide. The exposure of the primed cells results in a subsequent increase in the fMLP response. Thus, primed cells are susceptible to the millimeter waves. Specific inhibitors of the protein kinase abolished the mm wave effect on the primed cells, what means that protein kinase participates in transduction of the mm wave signal.