Study type: Medical/biological study (experimental study)

Immunomodulating action of low intensity millimeter waves on primed neutrophils med./bio.

By: Safronova VG, Gabdoulkhakova AG, Santalov BF
Published in: Bioelectromagnetics 2002; 23 (8): 599-606
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Aim of study (acc. to author)

To examine the millimeter wave effects on primed and unprimed neutrophil and estimate the role of the protein kinases in these effects.

Background/further details

Isolated neutrophils were primed by a chemotactic peptide N-formyl-methionyl-leucyl-phenylalanine (fMLP, 10 nM for 20 min), and then activated by 1 µM fMLP.
Different sets of intact and treated cells were irradiated simultaneously: (1) intact cells and primed cells; (2) primed cells and the cells treated initially with one of the inhibitors and then 1 min later with the priming agent; (3) intact cells, primed cells, and the cells treated initially with one of the inhibitors and then 1 min later with the priming agent. All cells were activated with 1 µM fMLP.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 41.95 GHz
Modulation type: CW
Exposure duration: 20 min

Exposure 1

Main characteristics
Frequency 41.95 GHz
Type
Charakteristic
Exposure duration 20 min
Modulation
Modulation type CW
Exposure setup
Exposure source
Distance between exposed object and exposure source 0.4 m
Chamber Incubator C1241
Setup mini dishes with cells were placed in the mini-dish holders inside the incubator.
Additional info 12 dishes, 6 with irradiation placed inside the incubator in a sample holder fixed on a horizontal polyurethane partition with a thickness of 10 mm. A hole 40 mm in diameter made in the partition exactly over the antenna. A holder with non-irradiated dishes was located 80 mm from the sample holder.
Parameters
Measurand Value Type Method Mass Remarks
SAR 450 µW/g unspecified calculated unspecified at an incident power density of 19.5 µW/cm²
power 7.5 mW unspecified - - 19.5 µW/cm² corresponding incident power density at 400 mm from the antenna
Additional parameter details

Incident power calculated as 150 µW/cm² at a distance of 400 mm from the antenna (far field) at the maximum power output of 58 mW.

Reference articles

  • Gapeyev AB et al. (1997): Inhibition of the production of reactive oxygen species in mouse peritoneal neutrophils by millimeter wave radiation in the near and far field zones of the radiator

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

Preliminary millimeter wave irradiation of the unprimed cells at 20°C did not act on the ROS production induced by the chemotactic peptide. The exposure of the primed cells results in a subsequent increase in the fMLP response. Thus, primed cells are susceptible to the millimeter waves. Specific inhibitors of the protein kinase abolished the mm wave effect on the primed cells, what means that protein kinase participates in transduction of the mm wave signal.

Study character:

Study funded by

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