Study type: Medical/biological study (experimental study)

Cytogenetic consequences of microwave irradiation on mammalian cells incubated in vitro med./bio.

By: Yao KT
Published in: J Hered 1982; 73 (2): 133-138
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Aim of study (acc. to editor)

To study cytogenetic consequences of microwave irradiation on mammalian cells incubated in vitro.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 2.45 GHz
Exposure duration: 7 days
  • SAR: 15.2 mW/g unspecified

Exposure 1

Main characteristics
Frequency 2.45 GHz
Type
Waveform
Exposure duration 7 days
Exposure setup
Exposure source
Chamber Microwave oven, six T-25 flasks
Setup T-25 flasks placed around a rubber cylinder placed in the middle of the oven
Parameters
Measurand Value Type Method Mass Remarks
SAR 15.2 mW/g unspecified estimated - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

Cell growth rate was significantly reduced after 7 or 15 subculture passages under exposition. Chromosome aberrations emerged after the cells had been microwave-irradiated for about 20 passages. The long-term exposition caused 0.84 chromosome breaks per cell in RH5 cells and 0.10 breaks per cell in RH16 cells. The number of chromosome breaks was greatly reduced in both cell cultures after the cells had been returned to the conventional incubator and maintained for 30 passages. The number of polyploid cells was increased during the irradiation, and was significantly reduced in the conventional incubator. Many RH5 cells lost one chromosome and became 10-chromosome cells (the number of 10-chromosome cells increased during irradiation and continued to increase after being returned to the conventional incubator).

Study character:

Study funded by