Study type: Medical/biological study (experimental study)

Magnetic field exposure saves rat cerebellar granule neurons from apoptosis in vitro. med./bio.

Published in: Neurosci Lett 2004; 365 (2): 83-86

Aim of study (acc. to author)

To study the effect of extremely low frequency magnetic field exposure on neuronal apoptosis.

Background/further details

Cerebellar granule neurons of postnatal rats were employed, which are known to undergo apoptosis under normal condition in vitro.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: continuous for 5 days
Exposure 2: 50 Hz
Exposure duration: continuous for 5 days
Exposure 3: 50 Hz
Exposure duration: continuous for 5 days

Exposure 1

Main characteristics
Frequency 50 Hz
Type
Waveform
Exposure duration continuous for 5 days
Exposure setup
Exposure source
  • not specified
Chamber Two chambers, one for exposure and the other for sham exposure maintained at 37°C
Setup 25 cm² plastic culture flasks were placed in the center of the chamber
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 300 mT unspecified unspecified - -
current density 1.6 A/m² maximum calculated - 1.0 A/m² (minimum value) induced in 25 cm² flask

Exposure 2

Main characteristics
Frequency 50 Hz
Type
Waveform
Exposure duration continuous for 5 days
Exposure setup
Exposure source
  • not specified
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
current density 2.8 A/m² maximum calculated - 2.0 A/m² (minimum value) induced in 75 cm² flask
magnetic flux density 300 mT unspecified unspecified - -

Exposure 3

Main characteristics
Frequency 50 Hz
Type
Waveform
Exposure duration continuous for 5 days
Exposure setup
Exposure source
  • not specified
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
current density 4.2 A/m² maximum calculated - 2.6 A/m² (minimum value) induced in 150 cm² flask
magnetic flux density 300 mT unspecified unspecified - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • during exposure
  • after exposure

Main outcome of study (acc. to author)

Exposure to a rotating extremely low frequency magnetic field for 5 days saved immature cerebellar granule neurons from apoptosis and promoted survival at the flux density of 300 mT, whereas no neuronal survival was observed for sham exposure. The survival-promoting effect of extremely low frequency magnetic fields occurred in a manner that depended on the size of culture flasks, indicating that induced current plays a role in this phenomenon.
These data imply that extremely low frequency magnetic fields may serve as a potential tool for manipulating neuronal death and/or survival.

Study character:

Study funded by

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