Study type: Medical/biological study (experimental study)

Exposure to Power Frequency Magnetic Fields Suppresses X-Ray-Induced Apoptosis Transiently in Ku80-Deficient xrs5 Cells. med./bio.

Published in: Biochem Biophys Res Commun 2002; 292 (2): 355-361

Aim of study (acc. to author)

To determine whether the in vitro exposure to extremely low frequency electromagnetic fields can affect cell growth, cell cycle, apoptosis and protein expression in CHO cells with and without initial X-rays radiation.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 60 Hz
Exposure duration: continuously for 24 h
Exposure 2: 60 Hz
Exposure duration: continuously for 5 h, 10 h, 24h

Exposure 1

Main characteristics
Frequency 60 Hz
Type
Exposure duration continuously for 24 h
Exposure setup
Exposure source
  • not specified
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 5 mT unspecified - unspecified -
cf. remarks - - - - pretreatment of cells with 0 - 8 Gray X-rays for 5 h

Exposure 2

Main characteristics
Frequency 60 Hz
Type
Exposure duration continuously for 5 h, 10 h, 24h
Exposure setup
Exposure source
Sham exposure A sham exposure was conducted.
Additional info the experiment was performed a) only with EMF exposure b) with X-ray pretreatment of 1 Gray for 5 h
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 5 mT unspecified - unspecified -

Reference articles

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

The exposure of both cell lines to extremely low frequency electromagnetic fields alone had no effect on cell growth, cell cycle, apoptosis and protein expression.
Electromagnetic field exposure of xrs5 cells for 5 hours after X-rays radiation significantly inhibited G1 phase induced by X-rays (1 Gy) and resulted in elevated bcl-2 expression.
A significant decrease in the protein expression of p53, phospho-p53, caspase-3 and p21 was observed in xrs5 cells when radiation by X-rays (8 Gy) was followed by exposure to electromagnetic fields.
Exposure of xrs5 cells to the electromagnetic fields for 10 hours following radiation significantly decreased X-ray-induced apoptosis. However, this effect was not found in CHO-K1 cells.
Exposure of xrs5 cells to 60 Hz extremely low frequency electromagnetic fields following radiation can affect cell cycle distribution and transiently suppress apoptosis by decreasing the levels of caspase-3, p21, p53 and phospho-p53 and by increasing bcl-2 expression.

Study character:

Study funded by

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