To determine whether the in vitro exposure to extremely low frequency electromagnetic fields can affect cell growth, cell cycle, apoptosis and protein expression in CHO cells with and without initial X-rays radiation.
Exposure duration: continuously for 24 h
Exposure duration: continuously for 5 h, 10 h, 24h
|Exposure duration||continuously for 24 h|
|Sham exposure||A sham exposure was conducted.|
|Exposure duration||continuously for 5 h, 10 h, 24h|
|magnetic flux density||5 mT||unspecified||-||unspecified||-|
The exposure of both cell lines to extremely low frequency electromagnetic fields alone had no effect on cell growth, cell cycle, apoptosis and protein expression.
Electromagnetic field exposure of xrs5 cells for 5 hours after X-rays radiation significantly inhibited G1 phase induced by X-rays (1 Gy) and resulted in elevated bcl-2 expression.
A significant decrease in the protein expression of p53, phospho-p53, caspase-3 and p21 was observed in xrs5 cells when radiation by X-rays (8 Gy) was followed by exposure to electromagnetic fields.
Exposure of xrs5 cells to the electromagnetic fields for 10 hours following radiation significantly decreased X-ray-induced apoptosis. However, this effect was not found in CHO-K1 cells.
Exposure of xrs5 cells to 60 Hz extremely low frequency electromagnetic fields following radiation can affect cell cycle distribution and transiently suppress apoptosis by decreasing the levels of caspase-3, p21, p53 and phospho-p53 and by increasing bcl-2 expression.