Study type: Medical/biological study (experimental study)

No effects of intermittent 50 Hz EMF on cytoplasmic free calcium and on the mitochondrial membrane potential in human diploid fibroblasts. med./bio.

Published in: Radiat Environ Biophys 2004; 43 (3): 203-207

Aim of study (acc. to author)

To test the hypothesis that DNA strand brakes induced by ELF-electromagnetic fields as observed in human diploid fibroblast cells meight be mediated by cellular changes of intracellular free calcium and/or mitochondrial membrane potential.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: intermittent 5 min on/10 min off for 15 hours or 7, 9, 11, 15, 17 hours

Exposure 1

Main characteristics
Frequency 50 Hz
Type
Waveform
Exposure duration intermittent 5 min on/10 min off for 15 hours or 7, 9, 11, 15, 17 hours
Exposure setup
Exposure source
  • coil(s)
  • see setup details
Setup two identical coil systems that were placed inside a commercial incubator to ensure constant environmental conditions (37°C, 5% CO2, 95% humidity) optimized to provide a homogeneous field at the area of the Petri dishes. Each coil system itself consisted of 4 subcoils (2 coils with 56 windings, 2 coils with 50 windings), which were placed inside a µ-metal box.
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 1 mT - measured - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

A significant increase of DNA strand brakes induced by ELF-EMF exposure was found. However, no increase of intracellular free calcium or changes in mitochondrial membrane potential could be detected. Therefore the results suggest that EMF-induced DNA strand breakes in human fibroblast are not mediated by the examined cellular processes in vitro.

Study character:

Study funded by

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