Study type: Medical/biological study (experimental study)

Intracellular Ca Mobilization and Beta-hexosaminidase Release Are Not Influenced by 60 Hz-electromagnetic Fields (EMF) in RBL 2H3 Cells. med./bio.

Published in: Korean J Physiol Pharmacol 2011; 15 (5): 313-317

Aim of study (acc. to author)

To study the effect of electromagnetic fields on intracellular Ca2+ mobilization (as universal messenger) stimulated by melittin (0.1-1 µM), ionomycin (1-100 nM) and thapsigargin (10-1000 nM) and on beta-hexosaminidase release in RBL-2H3 cells.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 60 Hz
Exposure duration: continuous for 4 h or 16 h

Exposure 1

Main characteristics
Frequency 60 Hz
Type
Exposure duration continuous for 4 h or 16 h
Exposure setup
Exposure source
Setup four square coils; exposure cage with three floors (top, middle, bottom); exposure system placed in an incubator with cooling system; magnetic shielding system made of ferrite material used for the exposure system; spatial variation of the magnetic field <3%
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 0.1 mT - - - -
magnetic flux density 1 mT - calibration - at the center of the middle floor

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

Exposure to the magnetic field (60 Hz, 0.1 or 1 mT) for 4 or 16 h did not produce any cytotoxic effects in RBL-2H3 cells. Melittin, ionomycin and thapsigargin each dose-dependently increased the intracellular Ca2+ concentration. The increase of intracellular Ca2+ induced by these three agents was not affected by magnetic field exposure for 4 or 16 h in RBL-2H3 cells.
Magnetic field exposure (60 Hz, 0.1 or 1 mT) for 4 or 16 h did not affect the basal or 1 µM melittin-induced beta-hexosaminidase release in these cells.
The data suggest that magnetic field exposure (60 Hz, 0.1 or 1 mT), which is the limit value of occupational exposure (cf. ICNIRP2010), had no influence on intracellular Ca2+ mobilization and cellular function (exocytosis) in RBL-2H3 cells.

Study character:

Study funded by

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