Study type: Medical/biological study (experimental study)

Cellular disorders induced by high magnetic fields med./bio.

By: Valiron O, Peris L, Rikken G, Schweitzer A, Saoudi Y, Remy C, Job D
Published in: J Magn Reson Imaging 2005; 22 (3): 334-340
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Aim of study (acc. to author)

To evaluate whether high static magnetic fields affect the cytoskeleton and cell organization in different types of mammalian cells, including fibroblasts, epithelial cells, and differentiating neurons.

Endpoint

Exposure

Exposure Parameters
Exposure 1:
Exposure duration: continuously for 30 min
Exposure 2:
Exposure duration: continuously for 60 min

Exposure 1

Main characteristics
Frequency
Type
Exposure duration continuously for 30 min
Exposure setup
Exposure source
Setup Exposed and control cells were processed simultaneously but the control cells remained located far from the magnet, 3-4 m from the center of M2 and M5 and 2-3 m from 7T Magnex
Additional info The superconducting magnet (7 T) and M2 magnet (0-17 T) provided horizontal field and M5 (0-13 T) magnet provided vertical field.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 17 T maximum unspecified - 15 T, 13 T, 10 T and 7 T

Exposure 2

Main characteristics
Frequency
Type
Exposure duration continuously for 60 min
Exposure setup
Exposure source
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 17 T maximum unspecified - 15 T, 13 T, 10 T and 7 T

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

Exposure to high magnetic fields affects the cytoskeleton, with deleterious effects on cell viability, organization, and differentiation.
Exposure over 10 Tesla in the case of cycling cells, and over 15 Tesla in the case of neurons, affected cell viability (and resulted in cell loss), apparently because of cell detachment from culture dishes. In the remaining adherent cells, the organization of actin assemblies was perturbed, and both cell adhesion and spreading were impaired. Moreover, in the case of neurons, magnetic field exposure induced growth cone retraction and delayed cell differentiation

Study character:

Study funded by