Study type: Medical/biological study (experimental study)

Effect of magnetic field on the fibronectin adsorption, cell attachment and proliferation on titanium surface med./bio.

Published in: Clin Oral Implants Res 2005; 16 (5): 557-562

Aim of study (acc. to author)

To study the effect of magnetic field on the adsorption of specific recombinant fibronectin peptide (hFNIII9-10) on the titanium surface and hFNIII9-10 mediated cell attachment and cell proliferation.

Background/further details

Fibronectin is a major extracellular matrix component used by many cell types as a substrate for attachment.

Endpoint

Exposure

Exposure Parameters
Exposure 1:
Exposure duration: continuously through the night
Exposure 2:
Exposure duration: continuously for 2 h

Exposure 1

Main characteristics
Frequency
Type
Exposure duration continuously through the night
Exposure setup
Exposure source
Setup Titanium disks (25 mm in diameter, 1 mm in thickness) were placed in acrylic boxes at various distances from the magnet (located at the bottom located) to obtain different magnetic fields. Control disks were placed in an identical acrylic box without the magnet.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 1 mT - measured - 2 mT, 3 mT, 5 mT, 7 mT and 10 mT

Exposure 2

Main characteristics
Frequency
Type
Exposure duration continuously for 2 h
Exposure setup
Exposure source
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 1 mT - measured - 2 mT, 3 mT, 5 mT, 7 mT and 10 mT

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

The amount of adsorbed hFNIII9-10 showed no significant difference between control (0 mT) and six experimental groups (1, 2, 3, 5, 7, 10 mT). However, TE-85 cells attached significantly higher in groups of 1, 2, 5, 10 mT than in control group. Cell attachment in groups of 3 and 7 mT showed no significant difference compared to control group. TE-85 cells were observed to attach through filopodia. 1 mT stimulated cells showed significantly higher cell proliferation than those in 2, 3, and 7 mT.
Magnetic fields under 10 mT did not influence fibronectin adsorption on the titanium surface. However, a significant effect was revealed on cell attachment and cell proliferation.

Study character:

Study funded by