To study the effect of magnetic field on the adsorption of specific recombinant fibronectin peptide (hFNIII9-10) on the titanium surface and hFNIII9-10 mediated cell attachment and cell proliferation.
|Setup||Titanium disks (25 mm in diameter, 1 mm in thickness) were placed in acrylic boxes at various distances from the magnet (located at the bottom located) to obtain different magnetic fields. Control disks were placed in an identical acrylic box without the magnet.|
The amount of adsorbed hFNIII9-10 showed no significant difference between control (0 mT) and six experimental groups (1, 2, 3, 5, 7, 10 mT). However, TE-85 cells attached significantly higher in groups of 1, 2, 5, 10 mT than in control group. Cell attachment in groups of 3 and 7 mT showed no significant difference compared to control group. TE-85 cells were observed to attach through filopodia. 1 mT stimulated cells showed significantly higher cell proliferation than those in 2, 3, and 7 mT.
Magnetic fields under 10 mT did not influence fibronectin adsorption on the titanium surface. However, a significant effect was revealed on cell attachment and cell proliferation.