Activities of two pineal gland enzymes (i.e. enzyme activity (pineal N-acetyltransferase and hydroxyindole-o-methyltransferase), both involved in the biosynthesis of melatonin, and pineal melatonin levels, and melatonin levels in bood serum were investigated during light and dark phase.
|Chamber||two identical windowless rooms with 10 in x 4.75 in x 4.5 in Plexiglass cages|
|Setup||the aminals; between the two top plates exposed and between the two bottom plates sham exposed|
|Additional info||cages with wire mesh floors in contact with ground plate of the exposure apparatus. EF strength mapped with a 10 cm Kaune disk probe with a 2.48 cm diameter center|
Electric field exposure had no effect on pineal melatonin level or enzymatic activity of N-acetyltransferase and hydroxyindole-o-methyltransferase, respectively. However, exposed animals exhibited lower serum melatonin levels at night as compared with sham-exposed animals.
These findings suggest that degradation or tissue uptake of melatonin is stimulated due to exposure.