Study type: Medical/biological study (experimental study)

Induction of RhoGAP and pathological changes characteristic of Alzheimer's disease by UAHFEMF discharge in rat brain. med./bio.

Published in: Curr Alzheimer Res 2005; 2 (5): 559-569

Aim of study (acc. to author)

This study was performed to determine whether the exposure to ultrasound associated with high frequency electromagnetic field (UAHFEMF) can influence the murine behavior and cause histopathological changes characteristic for Alzheimer's disease.

Background/further details

Eight male rats (average weight: 200-250 grams) and 80 male mice (average weight: 30 grams) were used.
The mice underwent the so called passive avoidance test to examine their behavioral changes. They were placed in the light chamber and then let the mice enter in the dark chamber to get an electric shock. Naturally mice flee into the dark chamber because of their inborn light phobic habit. 24 hours later this test was repeated by measuring the time between the been placed in the light chamber and entry the dark chamber. In this way a statement can be made about the avoidance of mice to re-enter the dark chamber und their loss of fear in the light chamber.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 27.12 MHz
Exposure duration: intermittent, max 30 min on/5 min off, less than 12 h/day, total exposure duration up to 2454 h (928 h, 1000 h, 1644 h or 2148 h)
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Exposure 1

Main characteristics
Frequency 27.12 MHz
Type
Exposure duration intermittent, max 30 min on/5 min off, less than 12 h/day, total exposure duration up to 2454 h (928 h, 1000 h, 1644 h or 2148 h)
Exposure setup
Exposure source
Chamber Exposure facility containing five compartments made of insulated plastic material. Each compartment contained 2 animals. The exposure facility was shielded with 1 mm thick grounded copper plate.
Additional info Animals exposed to considerably strong EMF that was tested with a 20 cm fluorescent lamp to make alight.
Parameters

No parameters are specified for this exposure.

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Investigated organ system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

The passive avoidance test was performed on six mice, and all showed signs of visual and auditory agnosia and loss of fear in the light chamber. The post mortal section revelaed dilated ventricles and dense amyloid deposition in hippocampal areas.
Neurodegenerative changes characteristic for Alzheimer's disease could be demonstrated by the histopathological examinations. Further investigations on rat brain proteins identified the RhoGTPase-Activating Protein (TAGAP) which might play an important role in Alzheimer's disease by its function in signal transduction pathway and/or Tau protein formation.

Study character:

Study funded by

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