Study type: Medical/biological study (experimental study)

Effects of a 50 Hz magnetic field on Dictyostelium discoideum (Protista). med./bio.

Published in: Bioelectromagnetics 2006; 27 (7): 528-534

Aim of study (acc. to author)

To study the effects of an extremely low frequency electromagnetic field (200 µT, 50 Hz, for 3 h or 24 h at 21°C) on the amoeba Dictyostelium discoideum (a protist).

Background/further details

To date there is scanty evidence of an effect of weak extremely low frequency electromagnetic fields on protists (single-cell eukaryotic organisms) in the literature. Protists respond directly to environmental stimuli, thus appearing as very suitable experimental systems.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: continuous for 3 or 24 h

Exposure 1

Main characteristics
Frequency 50 Hz
Type
Waveform
Exposure duration continuous for 3 or 24 h
Exposure setup
Exposure source
Setup The coil was directly wound on the sample holder (1.4 cm in radius). Samples were kept in a non working coil system for sham exposure. 3 h and 24 h control samples were kept outside the generator. Additionally, 24 h exposed samples were transferred to standard conditions for 24 h and their respective control was performed for 48 h.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 200 µT effective value calculated - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

A delay in the early phase of the differentiation was found in 3 h exposed cells, and a significant decrease in the fission rate appeared in 24 h exposed cells. The propionylcholinesterase activity was significantly lower in 3 h exposed cells than in the controls, whereas 24 h exposed cells exhibited an increase in this enzyme activity. However, such effects appeared to be transient, as the fission rate and propionylcholinesterase activity values returned to the respective control values after a 24 h stay under standard conditions.

Study character:

Study funded by

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