To study the effects of an extremely low frequency electromagnetic field (200 µT, 50 Hz, for 3 h or 24 h at 21°C) on the amoeba Dictyostelium discoideum (a protist).
To date there is scanty evidence of an effect of weak extremely low frequency electromagnetic fields on protists (single-cell eukaryotic organisms) in the literature. Protists respond directly to environmental stimuli, thus appearing as very suitable experimental systems.
| Exposure | Parameters |
|---|---|
|
Exposure 1:
50 Hz
Exposure duration:
continuous for 3 or 24 h
|
|
| Frequency | 50 Hz |
|---|---|
| Type | |
| Waveform | |
| Exposure duration | continuous for 3 or 24 h |
| Exposure source |
|
|---|---|
| Setup | The coil was directly wound on the sample holder (1.4 cm in radius). Samples were kept in a non working coil system for sham exposure. 3 h and 24 h control samples were kept outside the generator. Additionally, 24 h exposed samples were transferred to standard conditions for 24 h and their respective control was performed for 48 h. |
| Measurand | Value | Type | Method | Mass | Remarks |
|---|---|---|---|---|---|
| magnetic flux density | 200 µT | effective value | calculated | - | - |
A delay in the early phase of the differentiation was found in 3 h exposed cells, and a significant decrease in the fission rate appeared in 24 h exposed cells. The propionylcholinesterase activity was significantly lower in 3 h exposed cells than in the controls, whereas 24 h exposed cells exhibited an increase in this enzyme activity. However, such effects appeared to be transient, as the fission rate and propionylcholinesterase activity values returned to the respective control values after a 24 h stay under standard conditions.
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