Study type: Medical/biological study (experimental study)

Antiproliferative effect of millimeter radiation on human erythromyeloid leukemia cell line K562 in culture: ultrastructural- and metabolic-induced changes. med./bio.

Published in: Bioelectrochemistry 2007; 70 (2): 214-220

Aim of study (acc. to author)

To study the effects of millimeter wave irradiation on the K562 cell line in vitro.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 53.57–78.33 GHz
Exposure duration: repeated daily exposure, 1 h/day, every other day, for 7 days

Exposure 1

Main characteristics
Frequency 53.57–78.33 GHz
Type
Charakteristic
Exposure duration repeated daily exposure, 1 h/day, every other day, for 7 days
Modulation
Modulation type cf. additional info
Additional info

microwave noise generator diode

Exposure setup
Exposure source
Chamber The irradiation was performed inside an incubator (47 x 58 x 47 cm³) at 37 °C.
Setup The cells from a stock flask were seeded into culture dishes and irradiated from the bottom through a polystyrene base plate. The antenna was placed sufficiently near for the beam to cover the entire surface of the samples, but still at far field conditions.
Sham exposure A sham exposure was conducted.
Additional info Resonance phenomena due to reflections inside the incubator were excluded.
Parameters
Measurand Value Type Method Mass Remarks
power density 1 µW/cm² mean - - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

Millimeter wave exposure induced a noticeable inhibition of the cell proliferation that could be related to ultrastructural changes (e.g. increase in the number of mitochondria). Such effects brought the exposed cell system to lose the homeostasis (and resulted in a high metabolic state).
Kinetic of glucose metabolism demonstrated that the exposed cells enhanced the glycolytic aerobic pathway, indicating that such system need to produce an extra-bioenergy. Most of the ATP synthesized served probably to perform the above processes resulting in a significant decrease of the proliferation rate without significant cell death increment.

Study character:

Study funded by

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