|Chamber||Exposures were performed in a shielded room (made of 12.5-mm thick low-carbon steel sheets) on a standard clean bench for tissue culture, and metal surfaces surrounding the horn antenna were covered with MW-absorbing material to minimize reflections. MW generator, power meter, and spectrum analyzer were located outside. [Rojavin et al., 1997, 1998]|
|Setup||Cell cultures in the 15-mm wells of a standard 24-well plate, placed on top of the antenna, were exposed, one well at a time. Four corner wells of each plate were loaded with keratinocyte suspension. Two wells were exposed to MW and two were left as plate controls.|
|Sham exposure||A sham exposure was conducted.|
|Additional info||Each corner well was individually exposed or sham exposed for 15 or 30 min, and the total exposure duration for each plate was 60 or 120 min.|
A statistically significant increase in the intracellular level of interleukin-1-β was observed. Cell proliferation, adhesion to tissue culture plates, migration, and cytokine induced chemotaxis were not affected by exposure.