Study type: Medical/biological study (experimental study)

Reactions of keratinocytes to in vitro millimeter wave exposure. med./bio.

Published in: Bioelectromagnetics 2001; 22 (5): 358-364

Aim of study (acc. to author)

To investigate the effects of millimeter wave exposure to human keratinocytes in vitro.

Background/further details

Proliferation, adhesion, migration, and RANTES- and interleukin-1-β-induced chemotaxis as well as interleukin-1-β production in human HaCaT keratinocyte cell line were studied.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 61.2 GHz
Modulation type: CW
Exposure duration: continuous for 15 or 30 min, see add. information

Exposure 1

Main characteristics
Frequency 61.2 GHz
Type
Exposure duration continuous for 15 or 30 min, see add. information
Additional info ± 2.1 GHz
Modulation
Modulation type CW
Exposure setup
Exposure source
Chamber Exposures were performed in a shielded room (made of 12.5-mm thick low-carbon steel sheets) on a standard clean bench for tissue culture, and metal surfaces surrounding the horn antenna were covered with MW-absorbing material to minimize reflections. MW generator, power meter, and spectrum analyzer were located outside. [Rojavin et al., 1997, 1998]
Setup Cell cultures in the 15-mm wells of a standard 24-well plate, placed on top of the antenna, were exposed, one well at a time. Four corner wells of each plate were loaded with keratinocyte suspension. Two wells were exposed to MW and two were left as plate controls.
Sham exposure A sham exposure was conducted.
Additional info Each corner well was individually exposed or sham exposed for 15 or 30 min, and the total exposure duration for each plate was 60 or 120 min.
Parameters
Measurand Value Type Method Mass Remarks
power 20 mW - measured - output power
power density 29 mW/cm² - calculated - ± 2 mW/cm²
SAR 770 W/kg - measured - ± 42 W/kg

Reference articles

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

A statistically significant increase in the intracellular level of interleukin-1-β was observed. Cell proliferation, adhesion to tissue culture plates, migration, and cytokine induced chemotaxis were not affected by exposure.

Study character:

Study funded by

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