The result of a previous study (see publication 12091) that the exposure to 50 Hz magnetic field significantly protects against UV-induced embryo death should be further investigated concerning:
1) magnetic flux density dependency,
2) exposure time and
3) genetic dependency.
More than 1200, four days old embryos from genetically not related hens were used. Embryos were exposed for 10, 20, and 60 minutes prior to 60 minutes UVC radiation exposure.
| Exposure | Parameters |
|---|---|
|
Exposure 1:
50 Hz
Exposure duration:
10, 20 or 60 min
|
|
eggs were exposed to 60 min UV-C radiation with 1.7 mW/cm² after EMF exposure
| Frequency | 50 Hz |
|---|---|
| Type | |
| Exposure duration | 10, 20 or 60 min |
| Exposure source | |
|---|---|
| Setup | two pairs of Helmholtz coils; the horizontal pair with a diameter of 40 cm, the vertical one with a diameter of 42 cm; all coils with three seperate 30 turn windings of which two were used; the two windings were connected to a switch to allow the use as parallel or antiparallel (for sham exposure) |
| Sham exposure | A sham exposure was conducted. |
| Measurand | Value | Type | Method | Mass | Remarks |
|---|---|---|---|---|---|
| magnetic flux density | 0.01 mT | - | measured | - | - |
| magnetic flux density | 0.025 mT | - | measured | - | - |
| magnetic flux density | 0.05 mT | - | measured | - | - |
| magnetic flux density | 0.1 mT | - | measured | - | - |
| magnetic flux density | 0.2 mT | - | measured | - | - |
No significant effects of magnetic field exposure, irrespective of exposure time, magnetic flux density, or field direction was found on the survival rate of embryos. Neither any genetic dependency on sensitivity to magnetic field exposure could be revealed.
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