As the presence of more than one dental alloy in the oral cavity often causes direct currents and voltage the cellular apoptosis mechanisms on oral mucosa cancer cells exposed to electric fields should be investigated. Oxidation-reduction using amongst others free radical scavengers were characterized in particular.
|Pulse width||900 ms|
The electrical fields exposure to oral mucosa cancer cells, dose-dependently resulted in decreased cell proliferation, increased cell cycle arrest, and increased apoptosis. Furthermore, robust reactive oxygen species generation, increased expression of NADPH oxidase subunits as well as heat shock protein 70 was observed.
These results and the observation with free radical scavengers suggest that apoptosis in oral mucosa cancer cells is initated by reactive oxidative species generation in response to direct current electrical field exposure.