The brains were taken from adult male Sprague-Dawley rats (150-250 g). Kainic acid (glutamine like herbal agent) was applied to induce coherent network oscillations in the beta wave and gamma wave (15-100 Hz).
Exposure duration: intermittent, 1 s on/1 s off, repeated 5 times
Exposure duration: intermittent, 10 s on/30 s off, repeated 5 or 20 times
Exposure duration: not clearly indicated
|Distance between exposed object and exposure source||6 mm|
|Setup||Brain slices were placed in an interface recording chamber, perfused with artificial cerebrospinal fluid (aCSF), with the pyramidal layer perpendicular and the axis of the pyramidal cells' dendrites parallel to the electric field that was generated by chlorided silver electrodes, 2 mm in diameter and 45 mm long, located at the sides of the well, about 6 mm from the slice and parallel to the aCSF flow.|
|Additional info||Measurements were averaged over five repeats at each frequency and field strength.|
The direct current electric fields changed transmembrane potentials in hippocampal neuron somata by 0.18 mV per V/m applied. Alternating sinusoidal electric fields had smaller effects on transmembrane potentials. The transmembrane potential dropped as an exponential decay function of frequency. Concerning neuronal network oscillations, alternating current electric fields of higher or equal 6 V/m peak to peak shifted the gamma peak in the power spectrum to centre on the applied field frequency or a subharmonic. The ability of applied alternating current fields to alter gamma oscillations had a well-defined threshold.