Study type: Medical/biological study (experimental study)

Effects of extremely low-frequency-pulsed electromagnetic field on different-derived osteoblast-like cells. med./bio.

Published in: Electromagn Biol Med 2008; 27 (3): 298-311

Aim of study (acc. to author)

To study whether the effects of extremely low frequency pulsed electromagnetic fields on cell proliferation, cell cycle and cell differentiation are similar in different-derived osteoblast-like cells (primary cell culture and cell line).

Background/further details

The effects of magnetic fields on morphology, proliferation, differentiation and mineralization of different kinds of osteoblast cells are studied to elucidate the mechanisms of osteogenesis.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 48 Hz
Modulation type: pulsed
Exposure duration: continuous for 24 h or 48 h

General information

cells were treated in four ways: i) cells cultured for 24 h under PEMF ii) cells cultured for 24 h without PEMF, then exposed for another 24 h to PEMF iii) cells exposed to PEMF for 24 h, then cultured for another 24 h without PEMF iv) cells exposed to PEMF for 48 h

Exposure 1

Main characteristics
Frequency 48 Hz
Type
Waveform
  • rectangular
Exposure duration continuous for 24 h or 48 h
Modulation
Modulation type pulsed
Pulse width 13 ms
Repetition frequency 48 Hz
Pulse type monophasic
Exposure setup
Exposure source
Setup 160 turns of 2.06 mm enamel copper wire wound around a 350 mm long cylindrical tube made of epoxy resins with an inner diameter of 190 mm, so that two sub-coils were created; cells placed in the center of the solenoid that was inside an incubator
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 1.55 mT - measured - -

Reference articles

  • Kirschvink JL (1992): Uniform magnetic fields and double-wrapped coil systems: improved techniques for the design of bioelectromagnetic experiments.

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

1) MC3T3-E1 cells: Exposure to extremely low frequency pulsed electromagnetic field did not affect the cell number. The cell percentage of S phase and G2 phase decreased significantly after 24 h and 48 h. No alkaline phosphatase activity was detected.
2) Primary osteoblast cells: The cell number did not alter after 24 h continuous exposure, but increased greatly after 48 h. The cell percentage of S phase and G2 phase increased significantly after 24 h and decreased significantly after 48 h. The alkaline phosphatase activity decreased significantly after 24 h exposure and increased significantly after 48 h exposure.
In conclusion, in contrast to the MC3T3-E1 cell line, the primary osteoblast cells seemed to be more sensitive to the extremely low frequency-pulsed electromagnetic field.

Study character:

Study funded by

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