Effect of 1 mT Sinusoidal Electromagnetic Fields on Proliferation and Osteogenic Differentiation of Rat Bone Marrow Mesenchymal Stromal Cells
Published in: Bioelectromagnetics 2013; 34 (6): 453-464
Aim of study (acc. to author)
Bassen H et al.
ELF in vitro exposure systems for inducing uniform electric and magnetic fields in cell culture media
Time of investigation:
- during exposure
- after exposure
Main outcome of study (acc. to author)
After one and two weeks, the cell viability was decreased in the 50 Hz and 70 Hz samples in comparison to the other samples. However, exposure to 10 Hz, 30 Hz and 50 Hz, but not 70 Hz enhanced the proliferation of the cells compared to the sham exposed sample.
After one week, the protein expression of osteocalcin and bone morphogenetic protein-2 in the culture medium was significantly increased in the exposed samples compared to the values measured after one day. After two weeks, the values remained elevated.
The expression of the alkaline phosphatase and collagen type 1 was significantly increased in the 10 Hz sample after one week compared to all other samples, while after two weeks, these expression values were increased especially in the 50 Hz sample. The expression level of fibroblast growth factor-2 in the 10 Hz sample was significantly increased after two weeks of exposure compared to all other samples, while the expression level of fibroblast growth factor receptor-2 was significantly increased after one and two weeks in the 30 Hz sample.
After 3 weeks, in the 50 Hz sample, the percentage of mineralized areas and the percentage of osteocalcin/bone morphogenetic protein-2 positive cells were significantly higher than in the sham exposure, 10 Hz and 70 Hz samples. Regarding cell morphology, no difference among the samples was observed.
The authors conclude that magnetic field exposure to 10 Hz and 50 Hz may be a promising therapeutic approach in bone regenerative medicine and should be further examined.
Study funded by
National Natural Science Foundation (NSFC), China
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