Different cell lines were used for different endpoints. For positive controls, the cells were exposed to 2 Gy X rays (for cell growth), to bleomycin (for micronucleus formation), to ethylmethansulfonate (for HPRT assay), or to heat (for heat shock protein expression).
|Setup||35 cm high solenoid coil (outer diameter = 45 cm, inner diameter = 29 cm) with 96 turns of insulated copper wire; coil with cooling system placed inside an incubator; culture dishes stacked in four layers and positioned in the center of the coil in the 9.5 cm high exposure area of 10 cm diameter|
|Sham exposure||A sham exposure was conducted.|
The data did not detect any effects of the intermediate frequency magnetic fields on cell growth, comet assay, micronucleus formation, HPRT gene mutation, expression of phosphorylated Hsp27, or nuclear translocation of Hsp27, Hsp70 or Hsp105. In conclusion, the data indicate that exposure to an intermediate frequency magnetic field at 6.05 mT(rms-value) for 2 h does not cause detectable cellular genotoxicity, and does not induce detectable cellular stress.