The mutagenic effect was determined by the microsatellite analysis.
Three types of mutation were investigated: 1.) change in allele size, 2.) loss of heterozygous state (as a result of a deletion in one cell at the time of irradiation) and 3.) alleles imbalance (occuring several cells divisions after the radiation insult).
Cells were unexposed (control group), electromagnetic field exposed and co-exposed (electromagnetic field plus gamma radiation).
Exposure duration: 12 h continuous
|Exposure duration||12 h continuous|
|Setup||2 solenoids, 300 mm long with a diameter of 120 mm each, consisting of 400 turns of 1.5 mm copper wire with additional 52 turns of wire at either end of the coils, that could be energised seperately; uniformity of the field inside the solenoids > 99 %; cells placed in T25 flasks inside the solenoids|
|Sham exposure||A sham exposure was conducted.|
|magnetic flux density||1 mT||-||-||-||-|
The exposure of the cells to extremely low frequency electromagnetic field alone at a field strength of 1mT (50 Hz) for 12 hours showed a 3.75-fold increase in mutation induction compared with the unexposed control group.
The co-exposure revealed an increase in the mutagenic effect of 0.3 and 3 Gy gamma radiation. Moreover, the treatment with 0.3 Gy induced more than 10 times more mutations per unit dose than radiation with 3 Gy.
These results clarify not only that extremely low frequency electromagnetic field is mutagenic as a single agent but also that it can potentiate the mutagenicity of ionizing radiation.