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Medical/biological study (experimental study)

Microwave pretreatment can enhance tolerance of wheat seedlings to CdCl(2) stress.

Published in: Ecotoxicol Environ Saf 2011; 74 (4): 820-825

Aim of study (acc. to author)

The objectives of the study were 1) to determine the combined effects of microwave pretreatment and cadmium stress on wheat seedlings and 2) to evaluate the effect of seed pretreatment by microwaves on protecting wheat from cadmium (CdCl2) stress damage using plant morphological and physiological indices.
Background/further details: Seeds were exposed to microwaves for 0, 5, 10 and 15 s (each n=1000) and when the seedlings were 7 days old (with one fully expanded leave), they were treated with 150 µM CdCl2 solution for 10 days. On days 2, 4, 6, 8 and 10 leaves and roots were sampled for various analysis.

Endpoint

  • cadmium stress tolerance of wheat

Exposure

Exposure Parameters
Exposure 1: 2,450 MHz
Exposure duration: continuous for 5 s, 10 s or 15 s
Exposure 1
Main characteristics
Frequency 2,450 MHz
Type
Exposure duration continuous for 5 s, 10 s or 15 s
Exposure setup
Exposure source
Setup seeds placed in the center of the microwave board
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
power 700 W - - - -
power density 126 mW/cm² - - - -
Reference articles
  • Chen YP (2006): Microwave treatment of eight seconds protects cells of Isatis indigotica from enhanced UV-B radiation lesions.
Exposed system:
  • plant
  • wheat (Triticum aestivum L.)

Methods Endpoint/measurement parameters/methodology

Investigated material:

Main outcome of study (acc. to author)

CdCl2 treatment alone reduced plant height, root length, dry weight, ascorbate and glutathione concentrations and the enzyme activities of peroxidase, superoxide dismutase, catalase, ascorbate peroxidase, and enhanced the concentration of malondialdehyde, hydrogen peroxide and the production of superoxide when compared with the control.
However, seeds with microwave pretreatment for 5 or 10 s conferred a tolerance to cadmium stress in wheat seedlings by decreasing the concentration of malondialdehyde and hydrogen peroxide, the production of superoxide and increasing the enzyme activities of peroxidase, superoxide dismutase, catalase, ascorbate peroxidase and ascorbate and glutathione concentration. Therefore, antioxidative enzymes and antioxidative compounds may be involved in tolerance of wheat seedlings to cadmium stress. The findings also showed that the microwave exposure had a positive physiological effect on the growth and development of cadmium stressed seedlings (significant increase of root length and dry weight).
Study character:

Study funded by

  • Ministry of Education, China
  • Key Subject of Biochemistry and Molecular Biology of Henan Province, China
  • Opening Foundation of Key Laboratory of Resource Biology and Biotechnology in Western China (Northwest University), China

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