研究のタイプ: 医学/生物学の研究 (experimental study)

[マイクロ波前処理はコムギ芽苗のCdCl(2)ストレス耐性を高める] med./bio.

Microwave pretreatment can enhance tolerance of wheat seedlings to CdCl(2) stress.

掲載誌: Ecotoxicol Environ Saf 2011; 74 (4): 820-825

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研究目的(著者による)

The objectives of the study were 1) to determine the combined effects of microwave pretreatment and cadmium stress on wheat seedlings and 2) to evaluate the effect of seed pretreatment by microwaves on protecting wheat from cadmium (CdCl2) stress damage using plant morphological and physiological indices.

詳細情報

Seeds were exposed to microwaves for 0, 5, 10 and 15 s (each n=1000) and when the seedlings were 7 days old (with one fully expanded leave), they were treated with 150 µM CdCl2 solution for 10 days. On days 2, 4, 6, 8 and 10 leaves and roots were sampled for various analysis.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 2,450 MHz
ばく露時間: continuous for 5 s, 10 s or 15 s

ばく露1

主たる特性
周波数 2,450 MHz
タイプ
  • electromagnetic field
ばく露時間 continuous for 5 s, 10 s or 15 s
ばく露装置
ばく露の発生源/構造
  • microwave oven
ばく露装置の詳細 seeds placed in the center of the microwave board
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
電力 700 W - - - -
電力密度 126 mW/cm² - - - -

Reference articles

  • Chen YP (2006): [8秒間のマイクロ波処理はタイセイ(植物名)の細胞をUV-B放射傷害増悪から防護する]

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:

研究の主なアウトカム(著者による)

CdCl2 treatment alone reduced plant height, root length, dry weight, ascorbate and glutathione concentrations and the enzyme activities of peroxidase, superoxide dismutase, catalase, ascorbate peroxidase, and enhanced the concentration of malondialdehyde, hydrogen peroxide and the production of superoxide when compared with the control.
However, seeds with microwave pretreatment for 5 or 10 s conferred a tolerance to cadmium stress in wheat seedlings by decreasing the concentration of malondialdehyde and hydrogen peroxide, the production of superoxide and increasing the enzyme activities of peroxidase, superoxide dismutase, catalase, ascorbate peroxidase and ascorbate and glutathione concentration. Therefore, antioxidative enzymes and antioxidative compounds may be involved in tolerance of wheat seedlings to cadmium stress. The findings also showed that the microwave exposure had a positive physiological effect on the growth and development of cadmium stressed seedlings (significant increase of root length and dry weight).

研究の種別:

研究助成

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