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Medical/biological study (experimental study)

The role of fatty acids in anti-inflammatory effects of low-intensity extremely high-frequency electromagnetic radiation.

Published in: Bioelectromagnetics 2011; 32 (5): 388-395

Aim of study (acc. to author)

To study the effects of low-intensity extremely high frequency electromagnetic radiation (42.2 GHz) on the fatty acid composition of thymic cells and blood plasma in normal mice and in mice with peritoneal inflammation.
Background/further details: Inflammation was induced by intraperitoneal zymosan injection. Four groups of mice (each group n=2) were used: (1) sham exposure group (injected with physiological saline solution); (2) exposure group (injected with saline solution); (3) sham exposure group (with zymosan-induced peritoneal inflammation); (4) exposure group (with zymosan-induced peritoneal inflammation). The experiments were repeated three times. At 4 h after exposure/sham exposure the mice were decapitated.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 42.2 GHz
Modulation type: CW
Exposure duration: continuous for 20 min
General information
Mice were divided into four groups: i) sham exposure ii) RF exposure iii) induced inflammation + sham exposure iv) induced inflammation + RF exposure
Exposure 1
Main characteristics
Frequency 42.2 GHz
Type
Charakteristic
Exposure duration continuous for 20 min
Modulation
Modulation type CW
Exposure setup
Exposure source
Distance between exposed object and exposure source 300 mm
Setup animals kept in a 100 mm x 100 mm x 130 mm plastic container; multilayer absorbent placed between the animal container and the floor; antenna's major lobe width = 130 mm at 300 mm distance; exposure from the top
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
power 8 mW - measured - -
power density 0.1 mW/cm² - calculated - -
SAR 1.5 W/kg - calculated - at the container's surface
Additional parameter details
geomagnetic field: 45 µT +/- 3 µT
Reference articles
  • Gapeev AB et al. (2010): [Responses of thymocytes and splenocytes to low-intensity extremely high-frequency electromagnetic radiation in normal mice and in mice with systemic inflammation].
  • Gapeyev AB et al. (2009): Features of anti-inflammatory effects of modulated extremely high-frequency electromagnetic radiation.
  • Gapeyev AB et al. (2008): Anti-inflammatory effects of low-intensity extremely high-frequency electromagnetic radiation: frequency and power dependence.
  • Gapeev AB et al. (2002): [A study of absorption of energy of the extremely high frequency electromagnetic radiation in the rat skin by various dosimetric methods and approaches].
Exposed system:
  • animal
  • mouse/BALB/c
  • whole body

Methods Endpoint/measurement parameters/methodology

Investigated material:
Investigated organ system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

The data showed that the exposure of normal mice to low-intensity extremely high frequency electromagnetic radiation significantly increased the content of polyunsaturated fatty acids (eicosapentaenoic acid and docosapentaenoic acid) in thymic cells. Using the inflammation model, it was shown that the exposure of mice significantly increased the content of polyunsaturated fatty acids (dihomo-gamma-linolenic acid, arachidonic acid, eicosapentaenoic, docosapentaenoic, and docosahexaenoic) and reduced the content of monounsaturated fatty acids (palmitoleic acid and oleic acid) in thymic cells.
Changes in the fatty acid composition in the blood plasma were less pronounced and became manifest in an increase in the level of saturated fatty acids during the inflammation.
The authors conclude that the changes of fatty acid composition induced by low-intensity extremely high frequency electromagnetic radiation may be considered as a key element in the mechanisms of biological effects of radiation.
Study character:

Study funded by

  • Russian Foundation for Basic Research (RFBR), Russia

Related articles

  • Gapeyev AB et al. (2008): Anti-inflammatory effects of low-intensity extremely high-frequency electromagnetic radiation: frequency and power dependence.
  • Stankiewicz W et al. (2006): Immunotropic influence of 900 MHz microwave GSM signal on human blood immune cells activated in vitro.
  • Makar VR et al. (2006): Effect of cyclophosphamide and 61.22 GHz millimeter waves on T-cell, B-cell, and macrophage functions.
  • Lushnikov KV et al. (2005): Comparative Study of Anti-Inflammatory Effects of Low-Intensity Extremely High-Frequency Electromagnetic Radiation and Diclofenac on Footpad Edema in Mice
  • Lushnikov KV et al. (2004): Effects of low-intensity ultrahigh frequency electromagnetic radiation on inflammatory processes.
  • Makar V et al. (2003): Effect of millimeter waves on cyclophosphamide induced suppression of T cell functions.
  • Lushnikov KV et al. (2002): [Effects of extremely high-frequency electromagnetic radiation on the immune system and systemic regulation of homeostasis].
  • Lushnikov KV et al. (2001): [Effect of extremely high frequency electromagnetic radiation of low intensity on parameters of humoral immunity in healthy mice].