To investigate whether millimeter waves can modulate T cell recovery after suppression with cyclophosphamide (CPA), a commonly used anticancer drug.
Exposure duration: repeated daily exposure, 30 min/day, for 3 days
|Exposure duration||repeated daily exposure, 30 min/day, for 3 days|
|Additional info||± 0.2 GHz|
|Distance between exposed object and exposure source||5 mm|
|Setup||The animals were restrained in plastic tubes of 3.5 cm diameter in such a way that their nose stuck out of a 5-mm hole in the front end and was kept at a distance of 5 mm from the central part of the horn. The mice were irradiated on the nose area as reported earlier [Rojavin et al., 1997; Radzievsky et al., 2000].|
|Sham exposure||A sham exposure was conducted.|
|Additional info||Four groups of animals were used: naive control, CPA treated, CPA treated and sham exposed, and CPA treated and MW exposed. CPA (100 mg/kg) solution in physiological saline (0.5 ml) was administered i.p. just before exposure on the second day. All irradiation procedures were performed in a double blind manner.|
|power density||31 mW/cm²||peak value||measured||-||± 5 mW/cm²|
|SAR||622 W/kg||peak value||measured||-||±100 W/kg at the skin surface|
The results show that irradiation with millimeter waves at 42.2 GHz and a peak incident power density of 31 mW/cm² enhance T cell recovery through alteration in activation and effector functions of CD4+ T cells. Millimeter wave irradiation significantly augmented the proliferation recovery process of spleen cells suppressed by CPA. It was shown that CD4+ T cells play an important role in this process. Millimeter waves exposure restored the activation of anti-CD3 stimulated CD4+ T cells and enhanced interferon-gamma production by these cells. Millimeter wave exposure also restored tumor necrosis factor-α secretory activity by macrophages of immunosuppressed mice. When normal healthy mice were irradiated, any significant effect of millimeter waves on either proliferation activity or activation process in splenocytes could be observed.