研究のタイプ: 医学/生物学の研究 (experimental study)

[低強度の超高周波電磁放射の抗炎症作用における脂肪酸の役割] med./bio.

The role of fatty acids in anti-inflammatory effects of low-intensity extremely high-frequency electromagnetic radiation.

掲載誌: Bioelectromagnetics 2011; 32 (5): 388-395

目的と方法 :低レベルの超高周波電磁界(42.2 GHz、0.1 mW/cm2 、ばく露時間20分)の影響を、正常マウスおよび腹膜炎症があるマウスの胸腺細胞および血漿脂肪酸組成について調べた。結果 :正常マウスへのばく露により、胸腺細胞の多価不飽和脂肪酸(PUFA)(エイコサペンタエン酸およびドコサペンタエン酸)の濃度上昇が見られた。ザイモサン誘発腹膜炎症のモデルマウスを用いた場合、ばく露により胸腺細胞のPUFA(ジホモ-g-リノレン酸、アラキドン酸、エイコサペンタエン酸、ドコサペンタエン酸、ドコサペンタヘキ酸)の有意な濃度上昇、および一価不飽和脂肪酸(MUFA)(パルミトオレイン酸、オレイン酸)の濃度低下を示した。血漿中の脂肪酸組成変化は明白でなく、炎症時の飽和脂肪酸レベル上昇が見られた。結論 :我々のデータは、PUFAが外部から胸腺細胞に入ることによりMUFAに置き換わるという考えを支持する。PUFAの代謝物質は炎症免疫の反応に関係が深い脂質メッセンジャーであることを考慮して、我々は、細胞膜リン脂質におけるPUFA濃度上昇が超高周波電磁界の抗炎症作用の実現を促進すると想定する。

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研究目的(著者による)

To study the effects of low-intensity extremely high frequency electromagnetic radiation (42.2 GHz) on the fatty acid composition of thymic cells and blood plasma in normal mice and in mice with peritoneal inflammation.

詳細情報

Inflammation was induced by intraperitoneal zymosan injection. Four groups of mice (each group n=2) were used: (1) sham exposure group (injected with physiological saline solution); (2) exposure group (injected with saline solution); (3) sham exposure group (with zymosan-induced peritoneal inflammation); (4) exposure group (with zymosan-induced peritoneal inflammation). The experiments were repeated three times. At 4 h after exposure/sham exposure the mice were decapitated.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 42.2 GHz
Modulation type: CW
ばく露時間: continuous for 20 min

General information

Mice were divided into four groups: i) sham exposure ii) RF exposure iii) induced inflammation + sham exposure iv) induced inflammation + RF exposure

ばく露1

主たる特性
周波数 42.2 GHz
タイプ
  • electromagnetic field
特性
  • far field
ばく露時間 continuous for 20 min
Modulation
Modulation type CW
ばく露装置
ばく露の発生源/構造
Distance between exposed object and exposure source 300 mm
ばく露装置の詳細 animals kept in a 100 mm x 100 mm x 130 mm plastic container; multilayer absorbent placed between the animal container and the floor; antenna's major lobe width = 130 mm at 300 mm distance; exposure from the top
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
電力 8 mW - 測定値 - -
電力密度 0.1 mW/cm² - 計算値 - -
SAR 1.5 W/kg - 計算値 - at the container's surface

Reference articles

  • Gapeev AB et al. (2010): [正常マウスおよび全身性炎症マウスの胸腺細胞および脾細胞の低強度超高周波電磁放射に対する応答]
  • Gapeyev AB et al. (2009): [変調された超高周波電磁放射の抗炎症効果の特徴]
  • Gapeyev AB et al. (2008): [低レベルの超高周波電磁界放射の抗炎症作用:周波数および電力密度依存性]
  • Gapeev AB et al. (2002): [様々なドシメトリ法およびアプローチによるラット皮膚の超高周波電磁放射のエネルギー吸収に関する研究]

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
研究対象とした臓器系:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

The data showed that the exposure of normal mice to low-intensity extremely high frequency electromagnetic radiation significantly increased the content of polyunsaturated fatty acids (eicosapentaenoic acid and docosapentaenoic acid) in thymic cells. Using the inflammation model, it was shown that the exposure of mice significantly increased the content of polyunsaturated fatty acids (dihomo-gamma-linolenic acid, arachidonic acid, eicosapentaenoic, docosapentaenoic, and docosahexaenoic) and reduced the content of monounsaturated fatty acids (palmitoleic acid and oleic acid) in thymic cells.
Changes in the fatty acid composition in the blood plasma were less pronounced and became manifest in an increase in the level of saturated fatty acids during the inflammation.
The authors conclude that the changes of fatty acid composition induced by low-intensity extremely high frequency electromagnetic radiation may be considered as a key element in the mechanisms of biological effects of radiation.

研究の種別:

研究助成

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