Study type: Medical/biological study (experimental study)

Calcium efflux of plasma membrane vesicles exposed to ELF magnetic fields--test of a nuclear magnetic resonance interaction model. med./bio.

Published in: Bioelectromagnetics 2012; 33 (7): 535-542

Aim of study (acc. to author)

To study the effects of extremely weak 60 Hz magnetic fields on the transport of Ca2+ in a biological system consisting of highly purified plasma membrane vesicles.

Background/further details

The authors tested a proposed quantum mechanical model postulating that magnetic field induced polarization of hydrogen nuclei can elicit a biological effect (see Belova et al. 2007).

Endpoint

Exposure

Exposure Parameters
Exposure 1: 0–60 Hz
Exposure duration: continuous for 30 min

Exposure 1

Main characteristics
Frequency 0–60 Hz
Type
Exposure duration continuous for 30 min
Additional info static magnetic field and AC field applied simultaneously
Exposure setup
Exposure source
Setup exposures for vertical time-varying and static magnetic field were performed in a two-dimensional coil system; horizontal field generated by three coils consisting of 300 turns of 1 mm copper wire each, spaced 98 mm apart; electric current through the middle coil reduced by 58% to improve the homogeneity of the field; vertical field generated by two Helmholtz coils with a diameter of 346 mm and 740 turns of 1 mm copper wire, spaced 182 mm apart; coil system placed in an underground room, whose walls were build as a Faraday cage; two warm water baths shielded from the MF of the stirring motor and the heating device by a 1 cm thick 200 cm x 170 cm aluminum plate; twelve 1.5 ml tubes exposed simultaneously in a water bath in the exposure device
Sham exposure A sham exposure was conducted.
Additional info environmental low frequency field (40-1000 Hz): B=0.09 µT sham exposure done under the environmental static magnetic field of B=16 µT
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 26 µT - measured - vertical static magnetic field
magnetic flux density 6.3 µT maximum measured - AC field 0.7, 1.3, 2.1, 2.5, 3.9, 5.4, 6.3 µT

Reference articles

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • before exposure
  • after exposure

Main outcome of study (acc. to author)

The predictions of the investigated model that at a frequency of 60 Hz the biological effect under investigation would significantly be altered at the amplitudes of 1.3 and 3.9 µT could not be confirmed (i.e. no effects on calcium efflux under exposure).

Study character:

Study funded by

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