研究のタイプ: 医学/生物学の研究 (experimental study)

[ELF磁界にばく露した原形質膜小胞のカルシウム流出‐核磁気共鳴相互作用モデルの検証] med./bio.

Calcium efflux of plasma membrane vesicles exposed to ELF magnetic fields--test of a nuclear magnetic resonance interaction model.

掲載誌: Bioelectromagnetics 2012; 33 (7): 535-542

この実験研究は、ほうれん草の葉の細胞から作成した、高度に純化した原形質膜小胞におけるCa2+輸送に対する60H磁界の影響を調べ、Lednevが提案した量子メカニズムモデル(水素原子の核分極効果)を検証した。45Ca2+をトレーサとして、26 μTの静磁界および0.6-6.3 μTの60Hzのばく露を受けた小胞におけるカルシウムイオン流出の動態を測定した結果、Lednevのモデルで予測されるような現象は確認されなかったと報告している。

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研究目的(著者による)

To study the effects of extremely weak 60 Hz magnetic fields on the transport of Ca2+ in a biological system consisting of highly purified plasma membrane vesicles.

詳細情報

The authors tested a proposed quantum mechanical model postulating that magnetic field induced polarization of hydrogen nuclei can elicit a biological effect (see Belova et al. 2007).

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 0–60 Hz
ばく露時間: continuous for 30 min
  • 磁束密度: 26 µT (vertical static magnetic field)
  • 磁束密度: 6.3 µT maximum (AC field 0.7, 1.3, 2.1, 2.5, 3.9, 5.4, 6.3 µT)

ばく露1

主たる特性
周波数 0–60 Hz
タイプ
  • magnetic field
ばく露時間 continuous for 30 min
Additional information static magnetic field and AC field applied simultaneously
ばく露装置
ばく露の発生源/構造
ばく露装置の詳細 exposures for vertical time-varying and static magnetic field were performed in a two-dimensional coil system; horizontal field generated by three coils consisting of 300 turns of 1 mm copper wire each, spaced 98 mm apart; electric current through the middle coil reduced by 58% to improve the homogeneity of the field; vertical field generated by two Helmholtz coils with a diameter of 346 mm and 740 turns of 1 mm copper wire, spaced 182 mm apart; coil system placed in an underground room, whose walls were build as a Faraday cage; two warm water baths shielded from the MF of the stirring motor and the heating device by a 1 cm thick 200 cm x 170 cm aluminum plate; twelve 1.5 ml tubes exposed simultaneously in a water bath in the exposure device
Sham exposure A sham exposure was conducted.
Additional information environmental low frequency field (40-1000 Hz): B=0.09 µT sham exposure done under the environmental static magnetic field of B=16 µT
パラメータ
測定量 種別 Method Mass 備考
磁束密度 26 µT - 測定値 - vertical static magnetic field
磁束密度 6.3 µT maximum 測定値 - AC field 0.7, 1.3, 2.1, 2.5, 3.9, 5.4, 6.3 µT

Reference articles

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露前
  • ばく露後

研究の主なアウトカム(著者による)

The predictions of the investigated model that at a frequency of 60 Hz the biological effect under investigation would significantly be altered at the amplitudes of 1.3 and 3.9 µT could not be confirmed (i.e. no effects on calcium efflux under exposure).

研究の種別:

研究助成

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