Study type: Medical/biological study (experimental study)

Effects of electromagnetic fields on Reelin and Dab1 expression in the developing cerebral cortex. med./bio.

Published in: Neurol Sci 2014; 35 (8): 1243-1247

Aim of study (acc. to author)

To investigate the effects of exposure to a 50 Hz-magnetic field on total protein concentration as well as protein expression of reelin and Dab1 in the developing cortex of mice.

Background/further details

Reelin is a protein which helps to regulate processes of neural migration and positioning in the developing brain via cell-cell interactions. Disabled-1 (Dab1) is an important regulator of Reelin signalling.
Pregnant mice were divided into three groups (n=10 per group): 1.) cage control (embryos n=42), 2.) sham exposure group (embryos n=48) and 3.) exposure group (embryos n= 56). Exposure or sham exposure took place from gestational day 15-17. Dams were killed on gestational day 18 and cortices of embryos were collected for examinations.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: 5 hours/day for 3 consecutive days

Exposure 1

Main characteristics
Frequency 50 Hz
Type
Exposure duration 5 hours/day for 3 consecutive days
Exposure setup
Exposure source
Setup urban electric line adaptor 220 V and 10 A was used for minimizing the heat production, EMF emitter set including bobbin (80 x 10), wires and metal nucleuses was put in the bottom of hatchery machine in a metal lacuna, calibration of the exposure facility was performed to confirm uniform distribution of the magnetic field intensity where mice were kept, temperature and humidity were monitored continuously
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 0.5 mT - - - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

No significant differences between the groups were seen regarding the total protein concentration. Additionally, no significant differences in the protein expression were seen between the cage control and the sham exposed group. However, in cortices from the exposure group the protein expression of Reelin and Dab1 was significantly increased compared to the cage control and the sham exposed group.
The authors conclude that exposure to a 50 Hz-magnetic field could influence the protein expression of Reelin and Dab1 in the developing cortex of mice.

Study character:

Study funded by

Related articles