Reelin is a protein which helps to regulate processes of neural migration and positioning in the developing brain via cell-cell interactions. Disabled-1 (Dab1) is an important regulator of Reelin signalling.
Pregnant mice were divided into three groups (n=10 per group): 1.) cage control (embryos n=42), 2.) sham exposure group (embryos n=48) and 3.) exposure group (embryos n= 56). Exposure or sham exposure took place from gestational day 15-17. Dams were killed on gestational day 18 and cortices of embryos were collected for examinations.
Exposure duration: 5 hours/day for 3 consecutive days
|Exposure duration||5 hours/day for 3 consecutive days|
|Setup||urban electric line adaptor 220 V and 10 A was used for minimizing the heat production, EMF emitter set including bobbin (80 x 10), wires and metal nucleuses was put in the bottom of hatchery machine in a metal lacuna, calibration of the exposure facility was performed to confirm uniform distribution of the magnetic field intensity where mice were kept, temperature and humidity were monitored continuously|
|Sham exposure||A sham exposure was conducted.|
|magnetic flux density||0.5 mT||-||-||-||-|
No significant differences between the groups were seen regarding the total protein concentration. Additionally, no significant differences in the protein expression were seen between the cage control and the sham exposed group. However, in cortices from the exposure group the protein expression of Reelin and Dab1 was significantly increased compared to the cage control and the sham exposed group.
The authors conclude that exposure to a 50 Hz-magnetic field could influence the protein expression of Reelin and Dab1 in the developing cortex of mice.