研究のタイプ: 医学/生物学の研究 (experimental study)

[発達中の大脳皮質におけるリーリンおよびDab1発現に対する電磁界の影響] med./bio.

Effects of electromagnetic fields on Reelin and Dab1 expression in the developing cerebral cortex.

掲載誌: Neurol Sci 2014; 35 (8): 1243-1247

この研究は、発達中の大脳皮質での総タンパク濃度(TPC)、リーリンおよびDab1発現に対する磁界の影響を調べた。神経上皮で作られた神経細胞放射状グリア線維に沿って皮質板に移動するが、カハールレチウス細胞が分泌するリーリンはこの移動に必須であり、大脳皮質の発生に重要な役割を果たす。30匹の妊娠したBalb/cマウスを10匹ずつの対照群磁界ばく露群、擬似磁界ばく露群に分けた。妊娠15日目のマウスをソレノイドコイル内に置き、約1 mT(50 Hz )に1日5時間、3日間連続でばく露した(15-17日目)。ばく露終了から24時間後に摘出した胎仔156匹(ばく露群56、擬似ばく露群48、対照群42)の大脳皮質組織標本のTPC、リーリンおよびDab1発現を測定した。その結果、ばく露群のTPCは、擬似ばく露群・対照群に比べ、有意に変化しなかった;ばく露群のリーリンおよびDab1発現は、擬似ばく露群・対照群に比べ、増加した、と報告している。

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研究目的(著者による)

To investigate the effects of exposure to a 50 Hz-magnetic field on total protein concentration as well as protein expression of reelin and Dab1 in the developing cortex of mice.

詳細情報

Reelin is a protein which helps to regulate processes of neural migration and positioning in the developing brain via cell-cell interactions. Disabled-1 (Dab1) is an important regulator of Reelin signalling.
Pregnant mice were divided into three groups (n=10 per group): 1.) cage control (embryos n=42), 2.) sham exposure group (embryos n=48) and 3.) exposure group (embryos n= 56). Exposure or sham exposure took place from gestational day 15-17. Dams were killed on gestational day 18 and cortices of embryos were collected for examinations.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 50 Hz
ばく露時間: 5 hours/day for 3 consecutive days

ばく露1

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
ばく露時間 5 hours/day for 3 consecutive days
ばく露装置
ばく露の発生源/構造
ばく露装置の詳細 urban electric line adaptor 220 V and 10 A was used for minimizing the heat production, EMF emitter set including bobbin (80 x 10), wires and metal nucleuses was put in the bottom of hatchery machine in a metal lacuna, calibration of the exposure facility was performed to confirm uniform distribution of the magnetic field intensity where mice were kept, temperature and humidity were monitored continuously
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
磁束密度 0.5 mT - - - -

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

No significant differences between the groups were seen regarding the total protein concentration. Additionally, no significant differences in the protein expression were seen between the cage control and the sham exposed group. However, in cortices from the exposure group the protein expression of Reelin and Dab1 was significantly increased compared to the cage control and the sham exposed group.
The authors conclude that exposure to a 50 Hz-magnetic field could influence the protein expression of Reelin and Dab1 in the developing cortex of mice.

研究の種別:

研究助成

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