To investigate the effects of puerarin on the gene expression and protein expression of the matrix metalloproteinase-2 in human fetal scleral fibroblasts exposed to extremely low frequency magnetic fields.
Puerarin is a compound found in plants (e. g. Puerariae lobatae) and belongs to the isoflavones. Herbs containing puerarin are used in the traditional Chinese medicine against various diseases.
Cell cultures were grown under different conditions: 1.) no treatment (control group), 2.) magnetic field exposure, 3.) magnetic field exposure + 0.1 µM puerarin, 4.) magnetic field exposure + 1 µM puerarin and 5.) magnetic field exposure + 10 µM puerarin.
Exposure duration: continuous for 24 hours
|Setup||coils (16 cm length, 14 cm width, 8 cm hight) consisted of 168 turns of copper wire, respectively; coils were placed in cell culture incubator and connected to the power outlet one hour before the experiment to generate a stable magnetic field; temperature 37°C ± 0.2°C|
|Sham exposure||A sham exposure was conducted.|
|magnetic flux density||0.2 mT||-||measured||-||-|
The gene expression and the protein expression of the matrix metalloproteinase-2 were significantly increased in exposed cell cultures without puerarin compared to the control group. An addition of puerarin significantly decreased the gene expression and protein expression in comparison to the exposure group without puerarin. The more puerarin was added to the culture medium the more were the expression levels decreased.
The authors conclude that exposure to extremely low frequency magnetic fields could increase the gene expression and protein expression in human fetal scleral fibroblasts and that puerarin could attenuate these effects.