Study type: Medical/biological study (experimental study)

Effect of puerarin on matrix metalloproteinase-2 in human fetal scleral fibroblasts treated with low frequency electromagnetic fields. med./bio.

Published in: J Tradit Chin Med 2013; 33 (5): 664-668

Aim of study (acc. to author)

To investigate the effects of puerarin on the gene expression and protein expression of the matrix metalloproteinase-2 in human fetal scleral fibroblasts exposed to extremely low frequency magnetic fields.

Background/further details

Puerarin is a compound found in plants (e. g. Puerariae lobatae) and belongs to the isoflavones. Herbs containing puerarin are used in the traditional Chinese medicine against various diseases.
Cell cultures were grown under different conditions: 1.) no treatment (control group), 2.) magnetic field exposure, 3.) magnetic field exposure + 0.1 µM puerarin, 4.) magnetic field exposure + 1 µM puerarin and 5.) magnetic field exposure + 10 µM puerarin.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: continuous for 24 hours

Exposure 1

Main characteristics
Frequency 50 Hz
Type
Waveform
Exposure duration continuous for 24 hours
Exposure setup
Exposure source
Setup coils (16 cm length, 14 cm width, 8 cm hight) consisted of 168 turns of copper wire, respectively; coils were placed in cell culture incubator and connected to the power outlet one hour before the experiment to generate a stable magnetic field; temperature 37°C ± 0.2°C
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 0.2 mT - measured - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

The gene expression and the protein expression of the matrix metalloproteinase-2 were significantly increased in exposed cell cultures without puerarin compared to the control group. An addition of puerarin significantly decreased the gene expression and protein expression in comparison to the exposure group without puerarin. The more puerarin was added to the culture medium the more were the expression levels decreased.
The authors conclude that exposure to extremely low frequency magnetic fields could increase the gene expression and protein expression in human fetal scleral fibroblasts and that puerarin could attenuate these effects.

Study character:

Study funded by

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