Study type: Medical/biological study (experimental study)

Effect of Extremely Low Frequency Electromagnetic Field and GABAA Receptors on Serum Testosterone Level of Male Rats. med./bio.

Published in: Int J Endocrinol Metab 2013; 11 (4): e11029

Aim of study (acc. to author)

To examine the effect of exposure to a 50 Hz-magnetic field alone or in combination with the GABAA antagonist bicuculline or the GABAA agonist muscimol on the serum testosterone level of rats.

Background/further details

Gamma aminobutyric acid (GABAA) can influence the generation of steroid hormones (e.g. testosterone).
Rats were divided into 10 groups (n=5 per group): 1.) exposure + injection of saline solution, 2.) sham exposure + injection of saline solution, 3.) exposure + injection of bicuculline (1 mg/kg body weight), 4.) sham exposure + injection of bicuculline (1 mg/kg), 5.) exposure + injection of bicuculline (3 mg/kg), 6.) sham exposure + injection of bicuculline (3 mg/kg), 7.) exposure + injection of muscimol (0.5 mg/kg), 8.) sham exposure + injection of muscimol (0.5 mg/kg), 9.) exposure + injection of muscimol (2 mg/kg) and 10.) sham exposure + injection of muscimol (2 mg/kg).
Injection was performed after the exposure/sham exposure period. About 40 minutes after the injection, rats were killed and blood samples were taken.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: 8 hours/day for 30 days

Exposure 1

Main characteristics
Frequency 50 Hz
Type
Exposure duration 8 hours/day for 30 days
Exposure setup
Exposure source
Chamber wooden cage (70 x 120 x 30 cm)
Setup 3 coils (insulated 1 mm copper wire, 200 turns each) were wound around the outer surface at equal distance; coils were connected in parallel and sealed with adhesive bandage
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 0.5 mT - measured - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

No significant differences were observed between the exposed groups and the corresponding sham exposed groups.
The authors conclude that exposure to a 50 Hz-magnetic field alone or in combination with the GABAA antagonist bicuculline or the GABAA agonist muscimol has no influence on the serum testosterone level of rats.

Study character:

Study funded by

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