研究のタイプ: 医学／生物学の研究 (experimental study)

[雄ラットの血清テストステロンレベルに対する低周波電磁界およびGABAA受容体の影響] med./bio.

Effect of Extremely Low Frequency Electromagnetic Field and GABAA Receptors on Serum Testosterone Level of Male Rats

著者: Taherianfard M, Bahaddini A, Keshtkar S, Fazeli M, Shomali T
掲載誌: Int J Endocrinol Metab 2013; 11 (4): e11029
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この研究は、成獣の雄ラット50匹を用いて、血清テストステロンレベルに対する低周波磁界およびGABAA受容体の影響を調べた。ばく露群5群、擬似ばく露群5群の計10群に分け、それぞれの群にELF-EMF（50 Hz, 0.5 mT）のばく露（擬似ばく露）を1日8時間、30日間与えた。ばく露終了後に5種類の物質（生理食塩水、低濃度ビククリンメチオダイド、高濃度ビククリンメチオダイド、低濃度ムスシモールハイドロブロミド、高濃度ムスシモールハイドロブロミド）を、ばく露群、擬似ばく露群5組それぞれの個体に注入し、40分後に血清の検査を行った。その結果、擬似ばく露の生食投与群に比べ、ムスシモールハイドロブロミド投与によりばく露、擬似ばく露の両方共に血清テストステロンレベルが低下した；各物質の投与に対し、ばく露群、擬似ばく露群の血清テストステロンレベルに有意差はなかった、と報告している。

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研究目的（著者による）

To examine the effect of exposure to a 50 Hz-magnetic field alone or in combination with the GABAA antagonist bicuculline or the GABAA agonist muscimol on the serum testosterone level of rats.

詳細情報

Gamma aminobutyric acid (GABAA) can influence the generation of steroid hormones (e.g. testosterone).
Rats were divided into 10 groups (n=5 per group): 1.) exposure + injection of saline solution, 2.) sham exposure + injection of saline solution, 3.) exposure + injection of bicuculline (1 mg/kg body weight), 4.) sham exposure + injection of bicuculline (1 mg/kg), 5.) exposure + injection of bicuculline (3 mg/kg), 6.) sham exposure + injection of bicuculline (3 mg/kg), 7.) exposure + injection of muscimol (0.5 mg/kg), 8.) sham exposure + injection of muscimol (0.5 mg/kg), 9.) exposure + injection of muscimol (2 mg/kg) and 10.) sham exposure + injection of muscimol (2 mg/kg).
Injection was performed after the exposure/sham exposure period. About 40 minutes after the injection, rats were killed and blood samples were taken.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 50 Hz
ばく露時間: 8 hours/day for 30 days

ばく露1

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
ばく露時間 8 hours/day for 30 days
ばく露装置
ばく露の発生源／構造
チャンバの詳細 wooden cage (70 x 120 x 30 cm)
ばく露装置の詳細 3 coils (insulated 1 mm copper wire, 200 turns each) were wound around the outer surface at equal distance; coils were connected in parallel and sealed with adhesive bandage
Sham exposure A sham exposure was conducted.
パラメータ
測定量 値 種別 Method Mass 備考
磁束密度 0.5 mT - 測定値 - -
Additional parameter details

magnetic field was homogeneous in a zone of 21 cm distance from the transverse borders and 9 cm from the longitudinal borders, cage was placed in this area

ばく露を受けた生物:

方法 影響評価項目／測定パラメータ／方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム（著者による）

No significant differences were observed between the exposed groups and the corresponding sham exposed groups.
The authors conclude that exposure to a 50 Hz-magnetic field alone or in combination with the GABAA antagonist bicuculline or the GABAA agonist muscimol has no influence on the serum testosterone level of rats.

研究の種別:

研究助成

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