Study type: Medical/biological study (experimental study)

The synergic effects of Crocus sativus L. and low frequency electromagnetic field on VEGFR2 gene expression in human breast cancer cells. med./bio.

Published in: Avicenna J Med Biotechnol 2014; 6 (2): 123-127

Aim of study (acc. to author)

To investigate the potentially synergistic effects of saffron and extremely low frequency magnetic fields on gene expression of the vascular endothelial growth factor 2 (VEGFR2) in human breast cancer cells.

Background/further details

The vascular endothelial growth factor 2 is known to be involved in the angiogenesis. A reduced expression of this growth factor could inhibit the angiogenesis in tumors and therefore inhibit the tumor growth.
Human breast cancer cells were subjected to different treatments: 1.) cultivation without treatment (control group), 2.) cultivation in the presence of saffron (100, 200, 400 and 800 µg/ml), 3.) magnetic field exposure and 4.) magnetic field exposure + saffron (100, 200, 400 and 800 µg/ml).

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: 1 hour

Exposure 1

Main characteristics
Frequency 50 Hz
Type
Exposure duration 1 hour
Exposure setup
Exposure source
  • not specified
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 0.04 T - - - or 0.004 T? (contradictory statements)

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • before exposure
  • after exposure

Main outcome of study (acc. to author)

In magnetic field exposed cells as well as in cells cultivated in the presence of saffron, the gene expression level of the vascular endothelial growth factor was significantly decreased in comparison to the control group. A combination (co-exposure) of both treatments led to a further decrease in the expression level, which indicates a synergistic effect. No results are stated regarding cell viability and cell morphology.
The authors conclude that saffron as well as exposure to extremely low frequency magnetic fields could reduce the gene expression level of the vascular endothelial growth factor 2 in human breast cancer cells.

Study character:

Study funded by

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