研究のタイプ: 医学/生物学の研究 (experimental study)

[ヒト乳癌細胞におけるVEGFR2遺伝子発現に対するサフランと低周波電磁界の相乗的影響] med./bio.

The synergic effects of Crocus sativus L. and low frequency electromagnetic field on VEGFR2 gene expression in human breast cancer cells.

掲載誌: Avicenna J Med Biotechnol 2014; 6 (2): 123-127

この研究は、サフラン(学名Crocus Sativus L.)と低周波磁界(50 Hz, 0.004 T)の組合せばく露がMCF7細胞株での血管内皮増殖因子受容器(VEGFR2)の遺伝子発現に影響するか否かを調べた。種々の濃度のサフラン抽出液でMCF7細胞を処置した48時間後に、全てのフラスコに低周波磁界ばく露を与えた。VEGFR2の発現レベルをRT-PCRで分析した。その結果、サフラン抽出液処置群ではVEGER2の発現レベルが低下した;EMFばく露はサフランの血管新生抑制作用を増強させた、と報告している。

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研究目的(著者による)

To investigate the potentially synergistic effects of saffron and extremely low frequency magnetic fields on gene expression of the vascular endothelial growth factor 2 (VEGFR2) in human breast cancer cells.

詳細情報

The vascular endothelial growth factor 2 is known to be involved in the angiogenesis. A reduced expression of this growth factor could inhibit the angiogenesis in tumors and therefore inhibit the tumor growth.
Human breast cancer cells were subjected to different treatments: 1.) cultivation without treatment (control group), 2.) cultivation in the presence of saffron (100, 200, 400 and 800 µg/ml), 3.) magnetic field exposure and 4.) magnetic field exposure + saffron (100, 200, 400 and 800 µg/ml).

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 50 Hz
ばく露時間: 1 hour

ばく露1

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
ばく露時間 1 hour
ばく露装置
ばく露の発生源/構造
  • 詳細不明
パラメータ
測定量 種別 Method Mass 備考
磁束密度 0.04 T - - - or 0.004 T? (contradictory statements)

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露前
  • ばく露後

研究の主なアウトカム(著者による)

In magnetic field exposed cells as well as in cells cultivated in the presence of saffron, the gene expression level of the vascular endothelial growth factor was significantly decreased in comparison to the control group. A combination (co-exposure) of both treatments led to a further decrease in the expression level, which indicates a synergistic effect. No results are stated regarding cell viability and cell morphology.
The authors conclude that saffron as well as exposure to extremely low frequency magnetic fields could reduce the gene expression level of the vascular endothelial growth factor 2 in human breast cancer cells.

研究の種別:

研究助成

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