Study type: Medical/biological study (experimental study)

Light-dependent and -independent behavioral effects of extremely low frequency magnetic fields in a land snail are consistent with a parametric resonance mechanism med./bio.

Published in: Bioelectromagnetics 1997; 18 (3): 284-291

Aim of study (acc. to author)

To investigate light-dependent behavioral effects of extremely low frequency magnetic field exposure on opioid mediated analgesia in the land snail Capaea nemoralis.
The administration of SCH34826, a selective inhibitor of the enzyme enkaphilinase, caused an increased enkephalin activity, which results again in an enhanced response latency in the foot-lifting behaviour onto a thermal stimulus (applied by a hotplate).
q.v. publication 4768

Endpoint

Exposure

Exposure Parameters
Exposure 1: 0–30 Hz
Exposure duration: 15 min
Exposure 2: 0–60 Hz
Exposure duration: 15 min
Exposure 3: 0–120 Hz
Exposure duration: 15 min

General information

Four groups of snails were used: sham light, sham dark, expoed light and exposed dark.

Exposure 1

Main characteristics
Frequency 0–30 Hz
Type
Waveform
Exposure duration 15 min
Additional info Sinusoidal signal was superimposed on the DC signal.
Exposure setup
Exposure source
Setup 40 cm x 40 cm animal holder platform was positioned at the center of the x and y axis of the coils, 10 cm below the center of the z-coil.
Additional info Snails were exposed either in the presence or absense of light. In light conditions, snails were placed into translucent plastic containers made up of identical top and bottom halves (12 cm² x 5 cm high). In dark conditions, the top and bottom plastic containers were covered with black electric tape.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 39.1 µT - measured - DC magnetic field.
magnetic flux density 70.5 µT peak value measured - time varying magetic field.

Exposure 2

Main characteristics
Frequency 0–60 Hz
Type
Waveform
Exposure duration 15 min
Exposure setup
Exposure source
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 141 µT peak value measured - time varying magetic field.
magnetic flux density 78.1 µT - measured - DC magnetic field.

Exposure 3

Main characteristics
Frequency 0–120 Hz
Type
Waveform
Exposure duration 15 min
Exposure setup
Exposure source
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 282 µT peak value measured - time varying magetic field.
magnetic flux density 156.2 µT - measured - DC magnetic field.

Reference articles

  • Prato FS et al. (1996): Behavioural evidence that magnetic field effects in the land snail, Cepaea nemoralis, might not depend on magnetite or induced electric currents

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Investigated organ system:
Time of investigation:
  • during exposure

Main outcome of study (acc. to author)

Magnetic field exposure leads to a reduction of opioid-induced analgesia. Light exposure at the same time significantly enhanced this effect rather than MF-exposure in the dark. But the percentage of reduction in analgesia was found to be independent of the frequency of the applied magnetic fields. The latter observation suggest a direct magnetic field detecting mechanism.

Study character:

Study funded by

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