Analgesia is mediated by opioids, either exogenous (e.g. morphine) or endogenous (e.g. enkephalin, endorphin). It is assumed that ELF magnetic field exposure reduces this effect caused by opioids and light has a modulatory role. In order to investigate this issue the behaviour of land snails on thermal stimuli (i.e. foot-lifting response on hotplate) was examined under dark and light conditions. To specific biochemical pathways involved in opioid analgesia were surveyed in detail: 1) Nitric oxide (NO)/nitric oxide synthase (NOS) pathway including L-arginine. 2) Use of SCH34826, a specific inhibitor of the enzyme enkephalinase.
Exposure duration: 30 min
|Exposure duration||30 min|
|Setup||snails were placed in the center of the coil|
|Additional info||In the light condition (1.8 W/m²) a group of 10 snails was placed into translucent plastic containers made of identical top and bottom halves (12 cm², 5 cm high). For dark exposures the top and the bottom plastic containers were covered with black electrical tape. Once the snails were placed in the darkened containers, the two halves were taped togather.|
|magnetic flux density||141 µT||peak value||measured||-||-|
The results of this study suggest a general light dependency of the action of ELF magnetic fields. Furthermore nitric oxide (NO) seems to be involved in the mediation of the effects caused by the magnetic fields.