Study type: Medical/biological study (experimental study)

Effect of Extremely Low Frequency Electromagnetic Field and/or GABAB Receptors on Foot Shock-induced Aggression in Rats. med./bio.

Published in: Basic Clin Neurosci 2014; 5 (2): 169-172

Aim of study (acc. to author)

To examine the effects of extremely low frequency magnetic fields alone or in combination with a GABAB-agonist or -antagonist on aggression behavior in rats.

Background/further details

Aggression constitutes a serious clinical and social problem. Neurotransmitters such as gamma aminobutyric acid (GABA) play a key role in modulation of aggression in all mammalian species. On the other hand, strong magnetic fields may influence the central nervous system. Therefore, the possible correlation of magnetic field exposure and aggression as well as the role of GABAB receptors should be investigated.
10 groups of rats were examined (n= 5 per group): 1.) sham exposure + saline, 2.) exposure + saline, 3.) sham exposure + 100 mg of the GABAB antagonist CGP35348 per kg body weight, 4.) exposure + 100 mg/kg of CGP35348, 5.) sham exposure + 200 mg/kg CGP35348, 6.) exposure + 200 mg/kg CGP35348, 7.) sham exposure + 1.7 mg of the GABAB agonist baclofen per kg body weight, 8.) exposure + 1.7 mg/kg baclofen, 9.) sham exposure + 3 mg/kg baclofen and 10.) exposure + 3 mg/kg baclofen. After the exposure period, rats received the substances via intraperitoneal injection and were tested for aggression behavior for 20 minutes.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: continuous for 8 h/day for 30 days

Exposure 1

Main characteristics
Frequency 50 Hz
Type
Exposure duration continuous for 8 h/day for 30 days
Exposure setup
Exposure source
Setup the magnetic field chamber consisted of a 70 x 120 x 30 cm wooden cage; three coils of electrically insulated 1 mm copper wire with 200 turns each were wound around the outer surface at equal distance; coils were connected in parallel and sealed with adhesive bandage; electrical source was an autotransformer with the input of 50 Hz and 220 V; field was homogeneous in a zone of 21 cm distance from the transverse borders and 9 cm from the longitudinal borders
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 500 µT - measured - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

No significant differences were observed between magnetic field exposed (group 2) and sham exposed rats (group 1). Only rats exposed to magnetic fields and treated with 100 mg/kg CGP35348 (group 4) showed a significantly increased number of "lifted up threats" compared to magnetic field exposed rats with saline injection (group 2) and compared to sham exposed rats with 100 mg CGP35348 (group 3).
The authors conclude that extremely low frequency magnetic fields alone or in combination with a GABAB-agonist or -antagonist have no influence on the aggression behavior of rats in the used aggression model.

Study character:

Study funded by

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