MicroRNAs (miRNA) are small, non-coding RNA molecules, which play a critical role in gene regulation. In this study, miRNAs related to brain and human diseases were analyzed.
Eight groups of rats were examined (n=8, respectively): 1) adult (10 weeks old) sham exposed female, 2) young (3 weeks old) sham exposed female, 3) adult sham exposed male, 4) young sham exposed male, 5) adult magnetic field exposed female, 6) young magnetic field exposed female, 7) adult magnetic field exposed male and 8) young magnetic field exposed male.
Exposure duration: 4 hours/day on 60 consecutive days
|Chamber||respectively 4 rats in a polycarbonate cage (26 x 17 x 13 cm3)|
|Setup||Helmholtz coil apparatus was placed in a Faraday cage (70 x 65 x 65 cm3) to shield against electric fields components, electrical noise, and vibrations; diameter of coils was 42.75 cm and a gap of 21.375 cm; coils were made of electrically and thermally insulated copper wire (2.2 mm) and constisted of 160 turns; temperature was 22-24°C with no significant difference between experiments|
|Sham exposure||A sham exposure was conducted.|
|Additional info||geomagnetic field of 50 µT|
|magnetic flux density||1 mT||-||measured||-||± 0.02 mT|
In young exposed females (group 6), adult exposed males (group 7), and young exposed males (group 8), significant differences (down-regulation and up-regulation) in the expression of diverse miRNAs were found when compared to the corresponding sham exposure groups (groups 2, 3, 4).
The authors conclude that 50 Hz magnetic fields could influence the miRNA expression in the brain and blood of rats and that these altered expression levels differ depending on age and are differently down-regulated or up-regulated in blood and brain.