Study type: Medical/biological study (experimental study)

Evaluation of the neuroprotective effects of electromagnetic fields and coenzyme Q10 on hippocampal injury in mouse. med./bio.

Published in: J Cell Physiol 2019; 234 (10): 18720-18730

Aim of study (acc. to author)

The neuroprotective effects of co-exposure to a 50 Hz magnetic field and coenzyme Q10 in a mouse model of hippocampal injury should be investigated.

Background/further details

Hippocampal injury was induced in adult female mice, using an intraperitoneal injection of trimethyltin hydroxide (2.5 mg/kg). Afterwards, 36 mice were divided into the following groups: 1) exposure to the magnetic field for 1 week, 2) treatment with coenzyme Q10 (10 mg/kg) for 2 weeks, 3) co-exposure to the magnetic field for 1 week and coenzyme Q10 for 2 weeks, 4) sham exposure group (treatment with sesame oil (solvent of coenzyme Q10) for 2 weeks and magnetic field sham exposure for 1 week, 5) control group (only hippocampal injury), 6) negative control (healthy mice without treatment).

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: 7 hours/day for 1 week

Exposure 1

Main characteristics
Frequency 50 Hz
Type
Exposure duration 7 hours/day for 1 week
Exposure setup
Exposure source
  • not specified
Setup Electromagnetic field generator (details in exposure reference article)
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 5.9 mT - - - -

Reference articles

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Investigated organ system:
Time of investigation:
  • during exposure
  • after exposure

Main outcome of study (acc. to author)

In Morris water maze, the control group (hippocampus injury only; group 5) showed significant impairments compared to the negative control (healthy mice; group 6). Treatment with coenzyme Q10 (group 2) and co-exposure to the magnetic field and coenzyme Q10 (group 3), however, resulted in a significant memory improvement compared to the control group. In histopathology, the number of necrotic and apoptotic cells was significantly increased in the control group compared to the negative control, and it was significantly decreased in groups 2 and 3 compared to the control group. The Western blot showed a significant upregulation of apoptotic proteins Bax and procaspase 3 in the control group compared to the negative control. The protein expression of Bax and procaspase 3 was significantly decreased and Bcl-2 was significantly increased in all treatment groups (groups 1-3) compared to the control group.
The authors conclude that co-exposure to a 50 Hz magnetic field and coenzyme Q10 might have neuroprotective effects in a mouse model of hippocampal injury, while exposure to the magnetic field alone had no effects.

Study character:

Study funded by

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