Study type: Medical/biological study (experimental study)

Neuroprotective Effect of Low Frequency-Pulsed Electromagnetic Fields in Ischemic Stroke. med./bio.

Published in: Appl Biochem Biotechnol 2017; 181 (4): 1360-1371

Aim of study (acc. to author)

The neuroprotective effects and mechanisms of action of exposure of mice to a pulsed 60 Hz magnetic field after ischemic stroke should be investigated.

Background/further details

Ischemic strokes were experimentally induced in male mice. After the stroke, mice were divided into 2 groups (n=18 each): 1) exposure to the magnetic field and 2) sham exposure. Mice from each group were killed after 1, 3 and 14 days of exposure (remark EMF-Portal: respective numbers were not stated).

Endpoint

Exposure

Exposure Parameters
Exposure 1: 60 Hz
Exposure duration: intermittent for 6 h/day for up to 14 days

General information

exposure started within 30-40 min after experimentally induced stroke

Exposure 1

Main characteristics
Frequency 60 Hz
Type
Waveform
Exposure duration intermittent for 6 h/day for up to 14 days
Additional info no details on pulse modulation
Exposure setup
Exposure source
  • not specified
Setup mice could move freely during exposure
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 10 mT - calculated - -

Reference articles

  • Cho H et al. (2012): Neural stimulation on human bone marrow-derived mesenchymal stem cells by extremely low frequency electromagnetic fields.

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Investigated organ system:
Time of investigation:
  • before exposure
  • during exposure
  • after exposure

Main outcome of study (acc. to author)

Exposed mice had significantly longer rotarod times compared to sham exposed mice, indicating better motoric functions.
Previous studies suggested that BDNF and protein kinase C play a central role in neuroprotection, which was confirmed in this study by a significantly increased expression of BDNF, TrkB and phosphorylated protein kinase B in exposed mice compared to sham exposed mice after 3 and 14 days. This resulted further in a significant decrease of gene expression and protein expression of pro-apoptotic Bad, Bax, caspase 3 and a significant increase of anti-apoptotic Bcl-xL expression in the exposure group compared to the sham exposure group.
Moreover, the protein expression of inflammatory mediators MMP9 and IL-1β was significantly decreased in exposed mice compared to sham exposed mice after 3 and 14 days of exposure.
The authors conclude that exposure of mice to a pulsed 60 Hz magnetic field might have a neuroprotective effect after ischemic stroke via suppression of inflammation and apoptosis. They propose that low frequency pulsed electromagnetic fields may be beneficial as a treatment during recovery after ischemic stroke.

Study character:

Study funded by

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