To investigate the effects of extremely low frequency (ELF) pulsed magnetic fields (PMF) on opioid peptide gene expression and the signaling pathway controlling the corresponding gene transcription in rat myocardial cells.
Ventricular cardic myocytes were isolated from male Wistar rats.
| Exposure | Parameters |
|---|---|
|
Exposure 1:
50 Hz
Modulation type:
pulsed
Exposure duration:
0.5 h, 1 h, 4 h or 8 h
|
|
| Frequency | 50 Hz |
|---|---|
| Type | |
| Waveform |
|
| Exposure duration | 0.5 h, 1 h, 4 h or 8 h |
| Modulation type | pulsed |
|---|---|
| Rise time | 1.34 ms |
| Measurand | Value | Type | Method | Mass | Remarks |
|---|---|---|---|---|---|
| magnetic flux density | 1.74 mT | peak value | unspecified | - | - |
| electric field strength | 2 mV/m | maximum | estimated | - | induced electric field at the inner walls of the culture dish |
The mRNA level of the opioid peptide prodynorphin was found to be markedly increased in PMF exposed myocytes. A direct PMF exposure of isolated nuclei leads to an increased prodynorphin mRNA level as well. Prodynorphin gene transcription could not be affected by nuclear PMF exposure in the presence of specific protein kinase C (PKC) inhibitores. This effect was found to be independent of PKC isoenzymes and their cellular distribution. Synthesis and secretion of dynorphin B, another opioid peptid, was also increased by PMF exposure.
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