To study the effects of a 50 Hz extremely low frequency sinusoidal magnetic field on the gene expression cytokine receptors in human acute myeloid leukaemia cells in vitro.
The investigated cell surface cytokine receptors exert a wide range of biological and immunological functions, e.g. in the defense against pathogens.
Tetradecanoylphorbol acetate treated cells served as the positive control.
| Exposure | Parameters |
|---|---|
|
Exposure 1:
50 Hz
Exposure duration:
continuous for 0.5 h, 1 h, 2 h, 4 h and 8 h
|
|
|
Exposure 2:
50 Hz
Exposure duration:
continuous for 12 h, 24 h, 36 h, 48 h and 72 h
|
|
The field intensities were chosen because their similarity to occupational exposure levels.
| Frequency | 50 Hz |
|---|---|
| Type | |
| Waveform | |
| Exposure duration | continuous for 0.5 h, 1 h, 2 h, 4 h and 8 h |
| Exposure source |
|
|---|---|
| Setup | 3 groups of square copper coils (36 x 36 cm), spaced 18 cm apart from each other; the coils provided a very uniform magnetic field in the area of 10 x 10 x 10 cm³ where the cell cultures were located; the coils were placed in a µ-metal container. Sham and positive control cells treated with phorbol 12 myristate 13-acetate were placed in an identical incubator where the conditions were same as for the exposure groups but without magnetic field. Control and sham exposures took place for 8 h. |
| Measurand | Value | Type | Method | Mass | Remarks |
|---|---|---|---|---|---|
| magnetic flux density | 0.1 mT | unspecified | measured | - | - |
| magnetic flux density | 0.8 mT | unspecified | measured | - | - |
| Frequency | 50 Hz |
|---|---|
| Type | |
| Waveform | |
| Exposure duration | continuous for 12 h, 24 h, 36 h, 48 h and 72 h |
| Exposure source |
|
|---|---|
| Additional info | Control and sham exposures took place for 8 h and 72 h, respectively. |
| Measurand | Value | Type | Method | Mass | Remarks |
|---|---|---|---|---|---|
| magnetic flux density | 0.1 mT | unspecified | measured | - | - |
| magnetic flux density | 0.8 mT | unspecified | measured | - | - |
The magnetic field exposure at 0.1 and 0.8 mT for 72 hours increased gene expression levels of TNF-Rp75 and IL-6Rα in human cells. No significant change was observed in gene expression levels of TNF-Rp55 and TGFβR1.
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