Study type: Medical/biological study (experimental study)

Differential damage in bacterial cells by microwave radiation on the basis of cell wall structure. med./bio.

Published in: Appl Environ Microbiol 2000; 66 (5): 2243-2247

Aim of study (acc. to author)

To examine the mechanism of microbial cell inactivation by microwave heating along with the differences in the effects on gram-positive and -negative bacteria.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 2.45 GHz
Exposure duration: up to 80 s

Exposure 1

Main characteristics
Frequency 2.45 GHz
Type
Exposure duration up to 80 s
Exposure setup
Exposure source
Setup cells placed in the center of the oven
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
power 600 W maximum - - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • during exposure
  • after exposure

Main outcome of study (acc. to author)

Microwave radiation of cell suspensions resulted in a dramatic reduction of the viable counts as well as increases in the amounts of DNA and protein released from cells. No significant reduction of cell density was observed in either cell suspensions (it is believed that most of the bacterial cells inactivated remained unlysed). A severe damage on the surface of most E. coli cells was found, yet there was no significant change observed in the B. subtilis cells. Exposed E. coli cells were lysed in the presence of SDS, yet B. subtilis cells were resistent to SDS.

Study character:

Study funded by

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